摘要
【目的】探讨外源肌醇对番茄幼苗生理生化指标的影响及其耐高温胁迫的分子机制,为提高番茄的抗热性提供理论依据。【方法】以番茄品种‘NRP20-7’为试验材料,常温25℃和高温42℃分别喷施水和肌醇,观察各处理的表型变化并进行高光谱成像分析,测定各处理组的生理指标,并通过qRT-PCR方法分析热胁迫响应基因,抗氧化及ABA相关基因的表达。【结果】20 mmol/L肌醇处理能够有效缓解高温对番茄幼苗的伤害,降低叶片的热损伤指数,提高最大光化学效率,并显著提高光合色素含量。高温下外源肌醇显著提高番茄幼苗中脯氨酸、可溶性糖含量和过氧化物酶活性,并显著降低丙二醛、过氧化氢和超氧阴离子含量。外源肌醇能够提高热胁迫响应基因、抗氧化相关基因、抗坏血酸合成基因表达,降低热休克蛋白基因表达。外源肌醇通过调控脱落酸合成与信号通路影响体内ABA的含量水平参与响应高温胁迫。【结论】外源肌醇通过调节植物体内渗透调节能力、抗氧化酶活性、相关基因表达水平及脱落酸合成与信号通路等复杂的生物学过程来增强番茄对高温胁迫的耐受性。
[Objective]Studing the effect of exogenous myo-inositol on the physiological and biochemical indexes of tomato seedlings and the molecular mechanism of tolerance to high temperature stress will provide a theoretical basis for the improvement of heat resistance of tomato.[Methods]Tomato variety‘NRP20-7’was used as the test material,and water and myo-inositol were sprayed separately at normal temperature and high temperature,respectively.The phenotypic changes under treatments were analyzed by hyperspectral imaging,and the physiological indexes of each treatment group were analyzed.The expression of heat responsive genes,antioxidant,and ABA-related genes were analyzed by qRT-PCR.[Results]20 mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato seedlings,reduce the heat damage index of tomato leaves,increase the maximum photochemical efficiency(F_(v)/F_(m)),and significantly increased the content of photosynthetic pigments.Exogenous myo-inositol increased proline and soluble sugar contents and peroxidase activity,and decreased malondialdehyde,hydrogen peroxide,and superoxide anion contents in tomato seedlings at high temperature.Exogenous myo-inositol increased the expression of heat response genes,antioxidant-related genes,ascorbic acid synthesis genes,and decreased the expression of heat shock protein genes.In addition,exogenous myo-inositol responded to high temperature stress by regulating abscisic acid synthesis and signaling pathways to regulate the level of ABA in tomato seedlings.[Conclusion]Exogenous myo-inositol enhances tomato tolerance to high temperature stress by regulating complex biological processes in plants.
作者
陈艳
韩利源
杜子龙
朱栋幸
朱为民
张迎迎
CHEN Yan;HAN Liyuan;DU Zilong;ZHU Dongxing;ZHU Weimin;ZHANG Yingying(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai Key Laboratory of Facility Horticulture Technology,Facility Horticulture Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Shanghai Jinshan District Zhangyan Town Economic Development Service Center,Shanghai 201514,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第12期1856-1867,共12页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家重点研发计划项目“大白菜和番茄优质多抗新种质创制与应用”(2023YFD1201504)。
关键词
番茄
高温胁迫
肌醇
抗逆性
基因表达
tomato
high temperature stress
myo-inositol
stress resistance
gene expression
