摘要
以吡罗克酮乙醇胺盐(OCT)作为主要抑菌控油功效成分,利用糠秕马拉色菌抑菌圈、分子对接预测、供氢体还原型辅酶Ⅱ(NADPH)荧光测定方法考察在传统硫酸盐含硅油体系中不同的锌化合物对OCT的协同抑菌控油作用。结果表明,含0.5%OCT洗发产品对糠秕马拉色菌表现出高度敏感性,但锌化合物对OCT无协同增效作用,其中碳酸锌、月桂酸锌、蓖麻油酸锌反而起到拮抗作用。戊二醇、己二醇和癸二醇对OCT和葡萄糖酸锌组合物无正向增效作用。乳糖锌、PCA锌、苯酚磺酸锌在Docking score指标有较好的结果,蓖麻醇酸锌、月桂酸锌在MMGBSA dG Bind指标有较好的结果,说明其与I型5α-还原酶结合可能更稳定。供氢体还原型辅酶Ⅱ荧光测试中发现0.8%蓖麻醇酸锌(p<0.05)、0.8%酵母锌(p<0.001)对OCT起到显著协同增效作用,与分子对接理论预测有一定的相关性。实验表明锌化合物与OCT的协同复配机理主要是通过增强对5α-还原酶的抑制作用,而不是提高对马拉色菌的抑菌作用。无机锌和有机锌均具有显著的效果,特别是蓖麻醇酸锌和酵母锌。
Using piroctone olamine(OCT)as the antibacterial and oil-controlling component,the synergic antibacterial and oil-controlling effects of different zinc compounds on OCT in the traditional sulfate-containing silicone oil system are investigated by using the antibacterial zone of Malassezia furfur,molecular dock prediction and NADPH fluorescence determination methods.The results show that the hair products containing 0.5%OCT has high sensitivity to Malassezia furfur,but zinc compounds have no synergistic effect on OCT,and zinc carbonate,zinc laurate and zinc ricinoleate have antagonistic effect on OCT.Pentanediol,hexanediol and decanediol also have no positive synergistic effect on OCT and zinc gluconate compositions.Lactose zinc,PCA zinc and zinc phenolsulfonate have good results in Docking score index,and zinc ricinoleate and zinc laurate have good results in MMGBSA dG Bind index,indicating more stable binding with 5α-reductase I.Two zinc compounds,0.8%zinc ricinoleate(p<0.05)and 0.8%yeast zinc(p<0.001),show significant synergistic effect on OCT in the fluorescence test of hydrogen donor reduced coenzymeⅡ,and have a certain correlation with molecular docking prediction results.The results show that the synergistic compounding mechanism of zinc compounds with OCT is mainly through enhancing the inhibition of 5α-reductase,rather than enhancing the antibacterial effect on Malassezia furfur.Both inorganic zinc and organic zinc have significant effects,especially zinc ricinoleate and yeast zinc.
作者
林凤英
简嘉铭
马洁婷
丁明宁
曾双
LIN Fengying;JIAN Jiaming;MA Jieting;DING Mingning;ZENG Shuang(Guangzhou Fandao Network Technology Co.,Ltd.,Guangzhou 510663,China)
出处
《生物化工》
CAS
2024年第5期1-7,共7页
Biological Chemical Engineering
