摘要
十字花科作物主要由S位点的复等位基因控制自交不亲和性,羽衣甘蓝(Brassica oleracea var. acephala)是十字花科芸薹属中典型的自交不亲和性植物。本研究利用20对SSR引物首次构建了20个羽衣甘蓝自交系的指纹图谱,20对SSR引物共鉴定到65个等位变异,平均每对引物含有3.25个变异。等权重算术平均聚类法(UPGMA)将20个自交系聚类为3个遗传类群:OK1、OK2和OK3。其中遗传类群OK1的叶形为紧凑皱叶或披散皱叶,波浪状叶缘,叶色以红紫色为主;遗传类群OK2的叶形为光滑叶,波浪状或裂叶叶缘,叶色为白色外叶;遗传类群OK3的叶形为光滑叶,波浪状或裂叶叶缘,叶色以红紫色为主。利用SRK基因激酶区的特异性引物分析发现,20个羽衣甘蓝自交系中共有9个SRK单元型,分别是8个ClassⅠ型SRK单元型(SRK7、SRK13、SRK14、SRK16、SRK23、SRK36、SRK45和SRK51)和1个ClassⅡ型SRK单元型(SRK15)。遗传类群OK1主要为SRK15单元型(50%),其次为SRK23单元型(20%);遗传类群OK2主要为SRK15、SRK16单元型(均为40%);遗传类群OK3主要为SRK23单元型(40%)。亲和指数测定结果进一步验证了不同遗传类群内部或遗传类群间自交系杂交的亲和关系,杂交组合配制的过程中应兼顾遗传类群和SRK单元型。
Self-incompatibility in Cruciferous crop such as ornamental kale(Brassica oleracea var.acephala),is a critical reproductive barrier governed primarily by the multiple alleles at the S loci.Ornamental kale is a typical self-incompatibility member of this family.In this study,we constructed the fingerprint map of 20 inbred lines of ornamental kale using 20 pairs of SSR primers,identified a total of 65 allelic variations,averaging 3.25 variations per primer pair.The unweighted pair group method with arithmetic mean(UPGMA)clustered these into three district genetic groups:OK1,OK2,and OK3.Group OK1 is characterized by wrinkled,wavy leaves,with red and purple as the dominant colors.In contrast,group OK2 features smooth leaves that are either wavy or lobed,with white outer leaves.Group OK3 also presents smooth leaves,which may be wavy or lobed,with red and purple as the primary colors.Through the analysis of specific primers in the kinase region of SRK gene,nine SRK haplotypes were identified in these inbred lines including eight class I SRK haplotypes(SRK7,SRK13,SRK14,SRK16,SRK23,SRK36,SRK45,and SRK51)and one class II SRK haplotype(SRK15).The SRK15 haplotype was predominant in the group OK1(50%),followed by the SRK23 haplotype(20%).In group OK2,the SRK15 and SRK16 haplotype were both major(at 40%each).In group OK3,the SRK23 was predominant(40%).Further analysis of self-incompatible indices confirmed compatibility among inbred lines from the same or different genetic groups,emphasizing the importance of considering both genetic groups and SRK haplotypes in hybrid development.
作者
冯一了
陈文迪
张斌
任文静
韩风庆
陈立
方智远
杨丽梅
庄木
吕红豪
王勇
季家磊
张扬勇
FENG Yiliao;CHEN Wendi;ZHANG Bin;REN Wenjing;HAN Fengqing;CHEN Li;FANG Zhiyuan;YANG Limei;ZHUANG Mu;LYU Honghao;WANG Yong;JI Jialei;ZHANG Yangyong(College of Horticulture,Qingdao Agricultural University,Qingdao 266109,Shandong;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding,Beijing 100081)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2024年第11期1895-1906,共12页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(32172578)。
