摘要
本研究依托国家级自然保护区昆嵛山,分析其常见林型的土壤细菌群落结构与多样性,研究林分差异对土壤细菌群落的影响,为该区域森林健康和可持续发展提供理论支持,同时加深对植物与微生物之间相互作用的认识。选取赤松林(Pinus densiflora forest)、麻栎林(Quercus acutissima forest)、赤松-麻栎混交林、刺槐林(Robinia pseudoacacia forest)和杂木林为研究对象,以无林地为对照,利用16S rRNA基因高通量测序技术分析了6种林型下土壤细菌多样性、群落组成的差异,及其与土壤化学性质的关系。结果显示:变形菌门(Proteobacteria)是优势菌门,其次主要类群有酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)等。不同林型显著影响土壤细菌群落丰富度、均匀度指数,其中麻栎林土壤细菌多样性指数最高,混交林土壤细菌多样性指数最低,麻栎林土壤的ACE指数和Shannon指数均显著高于赤松林、杂木林、刺槐林和混交林。不同林型土壤细菌多样性变化可能与土壤pH值差异密切相关。NMDS和ANOSIM分析显示不同林型间土壤细菌群落结构具有显著差异(P <0.05),推断不同林型间植物凋落物及根系分泌物差异可能是驱动细菌群落变化的重要原因;此外,对环境因子与土壤细菌群落结构差异的关联分析显示,土壤全磷与有效磷含量可能是影响昆嵛山不同林型土壤细菌群落结构差异的关键环境因子。本研究从细菌多样性和群落结构对林型变化响应的角度,为探索温带森林生态系统健康管理提供了科学依据。
In present study,we investigated the community structure and diversity of soil bacteria in five common forest types in Kunyu Mountain,and explored the underlying mechanisms of effect of stand difference on soil bacteria,aiming to provide theoretical foundation for forest health recognition and sustainable forest management locally,and deepen the understanding on the interaction between plant and microorganism.Six common forest types in Kunyu Mountain,including Pinus densiflora,Quercusa acutissima,mixed P.densiflora-Q.cutissima,Robinia pseudoacacia,and miscellaneous broadleaved mixed forest were selected as the research objects,and the non-forest land was selected as the control.The community structure and diversity of soil bacteria under these forest types were analyzed using high-throughput sequencing of 16S rDNA genes,as well as the soil chemical properties.The results showed that,theαdiversity of soil flora in Q.acutissima forest,as well as in the control was significantly higher than that in other forest types,NMDS analysis showed there were significant differences in bacterial community structure among forest types(P<0.05).The three flora with the highest relative abundance at the phylum level were Proteobacteria,Acidobacteria,and Actinobacteria.The soil of mixed P.densiflora-Q.acutissima showed the lowest pH,while the miscellaneous forest showed obviously higher SOM(99.92 g/kg)and available nitrogen(317.24 mg/kg)than that of other forest types.The R.pseudoacacia forest showed the highest available phosphorus(78.8 mg/kg)among all forest types,and all forest types showed higher soil nutrients than the control.Canonical correlation analysis(CCA)indicated that the SOM,total phosphorus,and available phosphorus significantly impacted the bacterial community,and Mantel test further confirmed that the latter two factors took the main charge.Above all,our results revealed that,in Kunyu Mountain,the vegetation types had a significant impact on the soil bacterial community structure,and the soil total phosphorus and
作者
朱萍
刘文燕
刘展航
蒋博涵
许嘉庆
曲彦霖
孙中元
柏新富
侯玉平
ZHU Ping;LIU Wenyan;LIU Zhanhang;JIANG Bohan;XU Jiaqing;QU Yanlin;SUN Zhongyuan;BAI Xinfu;HOU Yuping(School of Life Sciences,Ludong University,Yantai 264025,China;Yantai Forest Resources Monitoring and Protection Service Center,Yantai 264001,China)
出处
《中山大学学报（自然科学版）（中英文）》
CAS
CSCD
北大核心
2024年第6期132-140,共9页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
烟台市科技创新发展计划项目(2022JCYJ029)
山东省自然科学基金(ZR2023MC077)。
关键词
土壤微生物
细菌多样性
群落结构
麻栎林
刺槐林
soil microbes
bacterial diversity
community structure
Quercusa acutissima forest
Robinia pseudoacacia forest
