摘要
目的分析颗粒酶B(GZMB)、程序性细胞死亡配体1(PD-L1)在非小细胞肺癌(NSCLC)组织中的表达情况及临床意义。方法收集2015年1月—2019年12月南阳市第一人民医院收治的105例NSCLC患者的癌及癌旁组织,采用免疫组化法测定GZMB、PD-L1表达,分析GZMB表达与患者临床病理特征的关系。选取2023年3—8月南阳市第一人民医院收集的30例NSCLC新鲜组织,采用RT-qPCR检测其GZMB、PD-L1 mRNA相对表达量。应用Spearman相关分析研究GZMB与PD-L1表达的相关性,绘制Kaplan-Meier曲线进行生存分析,采用Cox回归分析研究患者预后的影响因素。结果在NSCLC癌和癌旁组织中GZMB表达情况比较差异无统计学意义(P>0.05);相比于癌旁组织,PD-L1在癌组织中高表达(P<0.001)。Spearman相关分析显示,NSCLC组织中GZMB表达与PD-L1表达呈负相关关系(r=-0.448,P<0.001),且在NSCLC新鲜组织中GZMB与PD-L1 mRNA相对表达量呈负相关关系(r=-0.846,P<0.001)。GZMB的表达与NSCLC的浸润深度、淋巴结转移、远处转移有关(P<0.05)。105例NSCLC患者中位生存时间为39.36(1~100)个月,GZMB高表达组患者生存时间(59.15个月)长于低表达组(19.70个月),PD-L1高表达组患者生存时间(26.67个月)短于低表达组(50.46个月)。Cox回归分析显示,GZMB、PD-L1是影响NSCLC患者预后的独立因素(P<0.001)。结论NSCLC患者GZMB与PD-L1的表达呈负相关关系;GZMB高表达是患者预后良好的独立保护因素,PD-L1高表达是患者预后不良的独立危险因素。
Objective To analyze the expression and clinical significance of granzyme B(GZMB)and programmed cell death ligand 1(PD-L1)in non-small cell lung cancer(NSCLC).Methods Tumor and tumor-adjacent tissues were collected in 105 NSCLC patients from January 2015 to December 2019,and the expressions of GZMB and PD-L1 were detected to analyze the relationship between GZMB expression and clinicopathological features of NSCLC patients by immunohistochemistry.Thirty fresh NSCLC tissues collected from the First People's Hospital of Nanyang between March 2023 and August 2023,RT-qPCR was used to detect the relative expression levels of GZMB and PD-L1mRNA.GZMB and PD-L1 expression were performed by Spearman correlation analysis.Kaplan-Meier curve was drawn for survival analysis,and Cox regression was used to analyze the influencing factors of prognosis.Results There was no difference in the expression levels of GZMB between tumor and adjacent tissues in NSCLC patients(P>0.05),and PD-L1 was highly expressed in cancer tissues compared with para-cancer tissues(P<0.001).Spearman correlation analysis revealed a negative correlation between GZMB expression and PD-L1 expression in NSCLC tissues(r=-0.448,P<0.001).And showed a negative correlation between the relative mRNA expression levels of GZMB and PD-L1 in fresh NSCLC tissues(r=-0.846,P<0.001).The expression of GZMB was related to the depth of invasion,lymph node metastasis,and distant metastasis of NSCLC.The median survival time of 105 patients with NSCLC was 39.36 months(1-100 months),the survival time of patients with high expression of GZMB(59.15 months)was higher than that of patients with low expression(19.70 months),and the survival time of patients with high expression of PD-L1(26.67 months)was lower than that of patients with low expression(50.46 months).Cox regression analysis showed that GZMB and PD-L1 were independent factors affecting the prognosis of NSCLC patients(P<0.001).Conclusion In NSCLC patients,there is a negative correlation between the expression of GZMB and P
作者
孙奥龙
张丽柯
徐全晓
文鑫
李卫阳
SUN Aolong;ZHANG Like;XU Quanxiao;WEN Xin;LI Weiyang(Respiratory and Critical Care Medicine,the First People's Hospital of Nanyang City Affiliated to Xinxiang Medical College,Nanyang,Henan 473010,China;不详)
出处
《中华全科医学》
2024年第11期1863-1867,共5页
Chinese Journal of General Practice
基金
河南省医学科技攻关计划项目(LHGJ20200903)
南阳市科技攻关计划项目(23KJGG215)。
关键词
非小细胞肺癌
颗粒酶B
程序性细胞死亡配体1
相关性
预后
Non-small cell lung cancer
Granzyme B
Programmed cell death ligand 1
Correlation
Prognostic
