摘要
采用激光捕获显微切割(LCM)联合气质联用(GC-MS)技术探究挥发油在野生苍术不同节段根茎各组织(皮层、韧皮部、木质部和髓部)分泌腔的分布规律,并采取相同手段研究挥发油在栽培苍术不同移栽年限(1~3年)根茎各组织分泌腔的代谢特征。结果表明,在野生苍术不同节段的根茎中,以苍术素、苍术酮和白术内酯Ⅰ为主的挥发油成分在靠中部节段(第2~6节)根茎的皮层分泌腔的积累量显著高于两端节段(第1节和第7节)。在栽培苍术不同移栽年限根茎中,一年生时,苍术素、苍术酮、白术内酯Ⅰ和白术内酯Ⅱ等挥发油成分主要富集于皮层分泌腔;二年生时,除了芹烷二烯酮以外,其他挥发油成分在各组织分泌腔均有富集趋势;三年生时,以苍术酮、茅术醇和β-桉叶醇为主的挥发油成分主要倾向于富集在髓部分泌腔。整体上,栽培苍术各组织的挥发油积累量随着移栽年限增加而增多。研究通过非靶向代谢分析和绝对定量方法证实挥发油在野生苍术根茎中主要富集于中部节段的皮层分泌腔,在移栽苍术根茎中随着年限增加呈现由外往里“皮层-韧皮部-木质部-髓部”逐渐积累趋势,构建了苍术挥发油在根茎组织的时空分布图谱。
Laser capture microdissection(LCM)combined with gas chromatography-mass spectrometry(GC-MS)was employed to investigate the distribution of volatile compound in the secretory cavities of different tissues(cortex,phloem,xylem,and pith)in different rhizome sections of wild Atractylodes lancea.The same method was used to study the metabolic characteristics of the volatile compounds in the secretory cavities of different rhizome tissues of A.lancea transplanted for 1-3 years.In the rhizome of wild A.lancea,the accumulation of volatile compounds such as atractylodin,atractylon,and atractylenolideⅠin the secretory cavities of the cortex was significantly higher in the middle sections(sections 2-6)than that at the two ends(sections 1 and 7).In the rhizome of A.lancea transplanted for 1 year,the volatile compounds such as atractylodin,atractylon,atractylenolideⅠ,and atractylenolideⅡwere mainly accumulated in the secretory cavities of the cortex.In the rhizome of A.lancea transplanted for 2 years,the tested volatile compounds except selina-4(14),7(11)-dien-8-one were enriched in the secretory cavities of all the tissues.In the rhizome of A.lancea transplanted for 3 years,atractylon,hinesol,andβ-eudesmol were mainly accumulated in the secretory cavities of the pith.The overall accumulation of volatile compounds in each rhizome tissue of cultivated A.lancea increased with the increase in transplanting years.Non-targeted metabolomics and absolute quantification confirmed that the volatile compounds were mainly accumulated in the secretory cavities of the cortex in the middle rhizome sections of wild A.lancea.The accumulation in the rhizome of cultivated A.lancea presented a trend from cortex,phloem,xylem to pith over time.In this study,the spatio-temporal distribution map of volatile compounds in the rhizome of A.lancea was established.
作者
林怀斌
蒋待泉
王月枫
吕朝耕
彭政
郭秀芝
曹也
张成才
李阔
王升
郭兰萍
LIN Huai-bin;JIANG Dai-quan;WANG Yue-feng;YU Chao-geng;PENG Zheng;UO Xiu-zhi;CAO Ye;ZHANG Cheng-cai;LI Kuo;WANG Sheng;GUO Lan-ping(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Bejing 100700,China;Key Laboratory of Biology and Cultivation of Herb Medicine,Ministry of Agriculture and Rural Affairs,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第20期5422-5432,共11页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重大项目(81891014)
中国中医科学院科技创新工程项目(CI2021A03903,CI2021B013,CI2023E002)
国家重点研发计划项目(2023YFC3503801)
财政部和农业农村部国家现代农业产业技术体系项目(CARS-21)
中央本级重大增减支项目(2060302)。
关键词
苍术
分泌腔
挥发油
组织分布
激光捕获显微切割
气质联用
Atractylodes lancea
secretory cavity
volatile oil
tissue distribution
laser capture microdissection
gas chromatogra-phy-mass spectrometry
