摘要
目的探讨黄芪多糖(astragalus polysaccharide,APS)改善糖尿病(diabetes mellitus,DM)大鼠糖脂代谢的分子机制,以及其对无翅型小鼠乳房肿瘤病毒整合位点1(Wnt1)信号的调控机制。方法采用高脂饲料诱导构建大鼠DM模型,随机分为模型组(DM)、黄芪多糖低剂量组(APS-L)、黄芪多糖高剂量组(APS-H)、激动剂组(SB216763),每组10只,另取10只正常大鼠作为对照组(Control)。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记试剂盒(TUNEL)检测大鼠肝脏和胰腺组织中的细胞凋亡,酶联吸附反应试剂盒检测大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)的水平,5,5',6,6'-四氯-1,1',3,3'-四乙基苯丙咪唑羰花青碘化物(JC-1)染色检测胰腺组织中的线粒体膜电位,免疫荧光检测胰腺组织中葡萄糖转运子4(glucose transporter 4,GLUT4)和糖原合酶激酶3β(glycogen synthase kinase-3β,GSK-3β)的表达,油红O检测肝组织中的脂质沉积,免疫组化检测肝组织中脂肪酸合成酶(fatty acid synthase,FAS)和脂蛋白脂酶(lipoprotein lipase,LPL)的表达,Western blot实验检测大鼠肝组织中Wnt1、β-连环蛋白(β-catenin)、淋巴细胞瘤-2(B cell lymphoma-2,Bcl-2)和Bcl-2相关X蛋白(Bcl2-associated x protein,Bax)的表达。结果与Control组相比,DM组、APS-L组、APS-H组、SB216763组动物的空腹血糖(fasting blood glucose,FBG)、总胆固醇(total cholesterol,TC)和甘油三酯(triglyceride,TG)的含量,血清中MDA的水平,肝和胰腺组织中的细胞凋亡率,肝组织中脂质沉积,肝组织中Wnt1、β-catenin、Bax的表达均明显升高;胰腺组织中的线粒体膜电位,胰腺组织中GLUT4、GSK-3β的表达,肝组织中FAS、LPL、Bcl-2的表达均明显下降。与DM组大鼠相比,APS-L组、APS-H组、SB216763组大鼠FBG、TC、TG的含量,血清中MDA的水平,肝和胰腺组织中的细胞凋亡率,肝组织中脂质沉积,肝组织中Wnt1、β-catenin、Bax的表达明显下降;胰�
Objective To investigate the molecular mechanism of astragalus polysaccharide(APS)improving glucose and lipid metabolism in diabetes mellitus(DM)rats,and its regulation mechanism on wingless-type mouse mammary tumor virus integration site 1(Wnt1)signaling.Methods The rat DM model was induced by high-fat feed,which was randomly divided into a model group(DM),a low-dose astragalus polysaccharide group(APS-L),a high-dose astragalus polysaccharide group(APS-H),and an agonist group(SB216763),with 10 rats in each group,another 10 normal rats without modeling as the control group(Control).Terminal-deoxynucleoitidyl transferase-mediated nick end labeling(TUNEL)was used to detect the apoptosis of liver and pancreatic tissue of rats in each group.ELISA kit was used to detect the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in serum.5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimi dazol-carbocyanine iodide(JC-1)staining was used to detect the mitochondrial membrane potential in pancreatic tissue.Immunofluorescence was used to detect the expression of glucose transporter 4(GLUT4)and glycogen synthase kinase-3β(GSK-3β)in pancreatic tissue.Oil red O staining was used to detect lipid deposition in liver tissue.Immunohistochemistry was used to detect the expression of fatty acid synthase(FAS)and lipoprotein lipase(LPL)in liver tissue.Western blot was used to detect the expression of Wnt1,β-catenin,B cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax)in liver tissue.Results Compared with the control group,the fasting blood glucose(FBG),total cholesterol(TC),and triglyceride(TG)levels,the level of MDA in serum,the cell apoptosis rates in liver and pancreatic tissues,the lipid deposition in liver tissue,and the expression of Wnt1,β-catenin and Bax in liver tissue,in the DM group,APS-L group,APS-H group,and SB216763 group were significantly increased.While,the mitochondrial membrane potential in pancreatic tissue,the expression of GLUT4 and GSK-3 in pancreatic tissue,the expression of Fas and LPL,as well a
作者
武洁
刘旭光
冯晓辞
王英虎
李晓蕾
WU Jie;LIU Xuguang;FENG Xiaoci;WANG Yinghu;LI Xiaolei(Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang 050013,Hebei,China;Yuanshi County Hospital of Traditional Chinese Medicine,Yuanshi 051130,Hebei,China;Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang 050011,Hebei,China)
出处
《辽宁中医药大学学报》
CAS
2024年第11期43-51,共9页
Journal of Liaoning University of Traditional Chinese Medicine
基金
河北省中医药管理局课题(2021018)。
关键词
黄芪多糖
糖尿病
糖脂代谢
无翅型小鼠乳房肿瘤病毒整合位点1信号
astragalus polysaccharide
diabetes mellitus
glycolipid metabolism
wingless-type mouse mammary tumor virus integration site 1 signaling
