摘要
目的研究中国红豆杉内生真菌次生代谢产物及其生物活性。方法利用组织块法、划线法分离纯化内生菌,所得菌株采用分子鉴定法进行鉴定。采用大孔树脂柱、硅胶柱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用化学发光激酶检测试剂盒法、刃天青细胞活力测定法、酶标法测定磷脂酰肌醇3-激酶(PI3K)、磷酸二酯酶(PDE)、尖孢镰刀菌抑制活性。结果从中分离得到8种内生真菌,分别鉴定为木霉属、曲霉属、孢球托霉属、茎点霉属和青霉菌属真菌。菌株ZGG-5具有较强的PI3K抑制活性,IC_(50)值为(0.17±0.05)μg/mL,被鉴定为卵形孢球托霉。从ZGG-5中分离得到13个化合物,化合物13在1 mg/mL质量浓度下具有较强的PI3K抑制活性,抑制率为(97.8±0.51)%。结论中国红豆杉中存在具有PI3K有抑制活性的内生真菌。化合物1~12为首次从卵形孢球托霉中分离得到。
AIM To study the secondary metabolites from endophytic fungi of Taxus wallichiana var.chinensis(Pilger)Florin and their biological activities.METHODS Endophytes were isolated and purified by tissue block and scribing method,the obtained strains were identified by molecular method.Macroporous resin column and silica gel column were used for separation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The inhibition of phosphatidylinositol 3-kinase,phosphodiesterase and fusarium oxysporum activities were determined by chemiluminescence kinase assay kit method,resazurin cell viability assay and enzyme linked immunosorbent assay.RESULTS Eight endophytic fungi were isolated and identified as Trichoderma,Aspergillus,Gongronella,Phoma,Penicillium.Strain ZGG-5 had strong PI3K inhibitory activity with IC_(50)value of(0.17±0.05)μg/mL,and was identified as Gongronella butleri.Thirteen compounds were isolated from ZGG-5,and compound 13 had strong activities of inhibiting PI3K at concentration of 1 mg/mL,and the inhibitory rate was(97.8±0.51)%.CONCLUSION T.wallichiana var.chinensis contains endophytic fungi with strong activities of inhibiting PI3K.Compounds 1-12 are first isolated from G.butleri.
作者
陈蕾蕾
杨中铎
魏汉楠
马科贞
郭文林
张甜
CHEN Lei-lei;YANG Zhong-duo;WEI Han-nan;MA Ke-zhen;GUO Wen-lin;ZHANG Tian(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第10期3324-3329,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(22267011)
甘肃省高等学校产业支撑计划项目(2022CYZC-29)
温州市科技局项目(2022Y0872)。
关键词
中国红豆杉
内生真菌
卵形孢球托霉
次生代谢产物
分离鉴定
PI3K
Taxus wallichiana var.chinensis(Pilger)Florin
endophytic fungi
Gongronella butleri
secondary metabolites
isolation and identification
PI3K
