摘要
目的采用超高效液相色谱-串联质谱联用技术(UPLC-MS/MS)快速鉴定八角枫药材根和须根不同提取液的整体化学成分,筛选不同药用部位指标性成分。方法采用UPLC-MS/MS技术对八角枫根醇提液(ALE)、水提液(ALW)及须根醇提液(AFE)、水提液(AFW)进行全成分分析,应用主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)等多元统计分析,筛选不同药用部位八角枫药材中具有显著差异的化学成分并对其进行分析。结果从八角枫提取液中共鉴定出406个活性化学成分,总结了酚酸类、黄酮类、生物碱类、萜类等成分的质谱裂解规律,并在不同药用部位中筛选出秦皮苷、马钱子苷酸、亚苯甲酰基脲等20个指标性成分。结论八角枫不同药用部位及不同提取液所含的成分存在明显差异,差异化合物主要为酚酸类、黄酮类及生物碱类化合物,且主要富集在类黄酮生物合成途径。该研究为八角枫药效学物质基础阐明及八角枫不同部位品质差异提供了科学依据,并为八角枫药材的快速鉴定、质量控制与深入开发利用奠定了基础。
OBJECTIVE To idenntify the whole chemical components of different extracts of alangium chinense root and fibrous root using ultra high performance liquid chromatography-mass spectrometry(UPLC-MS/MS),and screen the index components of different medicinal parts.METHODS UPLC-MS/MS technology was used to analyze the total components of alcoholic extract(ALE),water extract(ALW)of Alangium chinense root,alcoholic extract(AFE)and water extract(AFW)of Alangium chinense fibrous root.Principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA)and other multivariate statistical analysis were used to screen and analyze the chemical components with significant differences in different medicinal parts of Alangium chinense.RESULTS A total of 406 active chemical components were identified from the extract of Alangium chinense.The mass spectrometry cracking rules of phenolic acids,flavonoids,alkaloids and terpenoids were summarized and analyzed in different medicinal parts.Twenty Index components such as fraxin,loganic acid and benzoyleneurea were screened from different medicinal parts.CONCLUSION There were obvious differences in the components of different medicinal parts and different extracts of Alangium chinense.Phenolic acids,flavonoids and alkaloids are the main compounds,and they were mainly concentrated in flavonoid biosynthesis pathway.This study provides a scientific basis for clarifying the pharmacodynamic material basis and quality differences of different parts of Alangium chinense,and lays a foundation for rapid identification,quality control and further development and utilization of Alangium chinense.
作者
王馨悦
刘畅
杨小英
牛梦伟
俸婷婷
石慧
周英
WANG Xinyue;LIU Chang;YANG Xiaoying;NIU Mengwei;FENG Tingting;SHI Hui;ZHOU Ying(Research Center for Application and Development of Medicinal and Food Resources,College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2024年第17期1605-1619,共15页
Chinese Pharmaceutical Journal
基金
贵州省科技计划项目资助(黔科合平台人才[2019]5407)
贵州省“十四五”中医药、民族医药重点学科项目资助[QZYYZDXK(JS)-2021-03]
贵州省教育厅2023年度自然科学研究项目资助(黔教技[2023]069号)
贵州省高层次创新型人才项目资助(黔科合平台人才-GCC[2023]047)。
