摘要
目的探讨血清T淋巴细胞激活性低分泌因子(RANTES)、白细胞介素-35(IL-35)对儿童急性阑尾炎伴穿孔的辅助诊断价值。方法选取2021年8月至2023年8月在唐山市丰南区医院就诊的132例急性阑尾炎患儿作为研究对象,根据是否发生阑尾穿孔分为穿孔组43例,未穿孔组89例。采用酶联免疫吸附试验检测血清RANTES、IL-35水平。采用Pearson相关分析血清RANTES、IL-35水平之间的相关性;采用多因素Logistic回归分析急性阑尾炎患儿发生阑尾穿孔的影响因素。采用受试者工作特征(ROC)曲线分析血清RANTES、IL-35对急性阑尾炎患儿阑尾穿孔的诊断效能。结果与未穿孔组相比,穿孔组的白细胞计数(WBC)、血小板计数(PLT)、中性粒细胞计数(NEUT)明显升高(P<0.05)。与未穿孔相比,穿孔组的血清RANTES水平明显升高(P<0.05),IL-35水平明显降低(P<0.05)。Pearson相关分析结果显示,急性阑尾炎患儿血清RANTES水平与IL-35水平呈负相关性(r=-0.474,P<0.05)。多因素Logistic回归分析结果显示,血清RANTES水平、WBC、NEUT、PLT升高是急性阑尾炎患儿发生阑尾穿孔的危险因素(P<0.05),IL-35水平升高是急性阑尾炎患儿发生阑尾穿孔的保护因素(P<0.05)。血清RANTES、IL-35联合诊断急性阑尾炎患儿阑尾穿孔的曲线下面积(AUC)优于RANTES、IL-35单独诊断的AUC(P<0.05)。结论急性阑尾炎患儿血清RANTES水平升高及IL-35水平降低与阑尾穿孔有关,二者联合检测可更好地辅助诊断急性阑尾炎患儿是否发生阑尾穿孔。
Objective To investigate the auxiliary diagnostic value of serum regulated on activation normal T cell expressed and secreted(RANTES)and interleukin-35(IL-35)in acute appendicitis with perforation in children.Methods A total of 132 children with acute appendicitis attending the Fengnan District Hospital in Tangshan City from August 2021 to August 2023 were selected as the study subjects,and they were divided into 43 cases in the perforated group and 89 cases in the non-perforated group according to whether or not perforation of the appendix had occurred.Serum RANTES and IL-35 levels were detected by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the correlation between serum RANTES level and IL-35 level.Multivariate Logistic regression was used to analyze the factors influencing the occurrence of appendiceal perforation in children with acute appendicitis.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic efficacy of serum RANTES and IL-35 for the occurrence of appendiceal perforation in children with acute appendicitis.Results Compared with the non-perforated group,the white blood cell count(WBC),platelet count(PLT)and neutrophil count(NEUT)in the perforated group were significantly higher(P<0.05).Compared with the non-perforated group,the RANTES level in the perforated group was significantly higher(P<0.05),while the IL-35 level was significantly lower(P<0.05).The results of Pearson′s correlation analysis showed that there was a negative correlation between serum RANTES level and IL-35 level in children with acute appendicitis(r=-0.474,P<0.05).Multivariate Logistic regression analysis showed that elevated RANTES level,WBC,NEUT and PLT were risk factors for the occurrence of appendiceal perforation in children with acute appendicitis(P<0.05),and elevated IL-35 level was a protective factor for the occurrence of appendiceal perforation in children with acute appendicitis(P<0.05).The area under the curve(AUC)of combined diagnosis of serum RANTES and IL-
作者
蔡晓艳
王佳欢
郭帅
吕佳佳
CAI Xiaoyan;WANG Jiahuan;GUO Shuai;LYU Jiajia(Department of Pediatrics,Fengnan District Hospital,Tangshan,Hebei 063300,China;Department of Pediatric Surgery,Shijiazhuang Maternal and Child Healthcare Hospital,Shijiazhuang,Hebei 050000,China;Department of Fetal Cardiac Ultrasound,Hebei Reproductive Maternity Hospital,Shijiazhuang,Hebei 050000,China;Department of Pediatrics,Shijiazhuang Maternal and Child Healthcare Hospital,Shijiazhuang,Hebei 050000,China)
出处
《检验医学与临床》
CAS
2024年第21期3191-3194,3200,共5页
Laboratory Medicine and Clinic
基金
河北省中医药管理局中医药类科学研究课题(2024133)。
关键词
T淋巴细胞激活性低分泌因子
白细胞介素-35
急性阑尾炎
穿孔
诊断
regulated on activation normal T cell expressed and secreted
interleukin-35
acute appendicitis
perforation
diagnosis
