摘要
目的建立电感耦合等离子体-串联质谱法(ICP-MS/MS)同时测定人血液和尿液中钴、镍、砷、钼、银、镉和铅的分析方法。方法血液以体积分数为0.10%的硝酸和体积分数为0.02%的曲拉通X-100混合溶液为稀释剂20倍稀释,尿液以体积分数为1.00%的硝酸溶液为稀释剂10倍稀释,采用钇-89、铑-103和镥-175为内标克服基体干扰,原位模式或质量转移模式克服质谱干扰,以ICP-MS/MS仪检测。结果7种重金属元素质量浓度在0.100~10.000μg/L时线性关系良好,相关系数均>0.9999。在血液和尿液中7种元素的检出限分别为0.003~0.021和0.003~0.031μg/L,定量下限分别为0.009~0.064和0.009~0.094μg/L,加标回收率分别为96.64%~102.90%和96.34%~104.50%,批内相对标准偏差(RSD)为0.32%~2.33%和0.25%~2.31%,批间RSD为1.07%~3.81%和1.30%~3.62%。样品在4℃条件下至少可保存14 d。结论ICP-MS/MS法简单、灵敏、准确,适用于职业病危害因素接触者血液和尿液中7种重金属元素的快速检测。
Objective To establish a method to simultaneously determinate cobalt,nickel,arsenic,molybdenum,silver,cadmium and lead in blood and urine using inductively coupled plasma tandem mass spectrometry(ICP-MS/MS).Methods The blood samples were diluted 20 times with a mixed solution of nitric acid(0.10%V/V)and Triton X-100(0.02%V/V).The urine samples were diluted 10 times with nitric acid(1.00%V/V).Yttrium-89,rhodium-103,and lutetium-175 were used as internal standards to decrease matrix interference,and either On-Mass mode or Mass-Shift mode was used to decrease mass spectrometry interference,with detection by ICP-MS/MS.Results The linear ranges of the seven elements were 0.100-10.000μg/L,with the correlation coefficient>0.9999.The detection limits for the seven elements in the blood and urine were 0.003-0.021 and 0.003-0.031μg/L,respectively,and the minimum quantification limits were 0.009-0.064and 0.009-0.094μg/L,respectively.The recovery rates were 96.64%-102.90%and 96.34%-104.50%for the blood and urine,respectively.The within-run relative standard deviations(RSDs)were 0.32%-2.33%and 0.25%-2.31%,and the between-run RSDs were 1.07%-3.81%and 1.30%-3.62%for blood and urine,respectively.The samples were stable at 4℃for at least 14 days.Conclusion ICP-MS/MS is a simple,sensitive,and accurate method for rapid detection of seven heavy metal elements in the blood and urine of occupational hazard-exposed workers.
作者
罗晓婷
梁聪敏
董明
吴邦华
邵军丽
LUO Xiaoting;LIANG Congmin;DONG Ming;WU Banghua;SHAO Junli(不详;Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangzhou,Guangdong 510300,China)
出处
《中国职业医学》
CAS
2024年第4期428-433,共6页
China Occupational Medicine
基金
广东省本科高校教学质量与教学改革工程建设项目(粤教高涵[2021]29号)
广东省医学科研基金(A2023058)
广东医科大学教学质量与教学改革工程项目(1JG21075)。
关键词
电感耦合等离子体-串联质谱
质谱干扰
血液
尿液
重金属元素
Inductively coupled plasma tandem mass spectrometry
Mass spectrum interference
Blood
Urine
Heavy metal element
