摘要
【目的】紫楠Phoebe sheareri幼苗根系不发达严重制约了紫楠幼苗的繁育,外源激素可以调节植物根系形态发育,用多效唑(PP_(333))和6-苄基腺嘌呤(6-BA)对紫楠幼苗进行处理,分析其对根系形态特征的影响,明确最适紫楠根系发育的激素及浓度。【方法】以同一母树上采集的成熟紫楠种子萌发的幼苗为试验材料,设置低、中、高3个浓度(PP_(333)浓度为0.6、6、60 mg·L^(-1);6-BA浓度为10、50、75 mg·L^(-1)),利用根系扫描仪统计紫楠幼苗主根长、主根直径、根系总长度及根系表面积等形态指标,分别分析2种外源激素对紫楠幼苗根系形态的影响。【结果】PP_(333)处理紫楠幼苗会影响紫楠的主根长、2级侧根数目和2级侧根总长度的增加,但对主根直径的增粗起到促进作用;6-BA处理会促进主根长、主根直径、根系总长度、根系总表面积、1级侧根数目和1级侧根总长度的增加,但限制2级侧根数目和2级侧根总长度的增加。PP_(333)和6-BA较CK组均促进拓扑指数增加,促进紫楠根系朝着鱼尾形分支结构进化。通过主成分分析和隶属函数分析,都得出在激素处理下,浓度为50 mg·L^(-1)的6-BA促进紫楠根系发育效果最好。【结论】不同激素类物质及同一激素的不同浓度对紫楠根系的主根、侧根生长有着不同的调控模式,选择50 mg·L^(-1)6-BA来促进紫楠根系的生长发育效果最好。
【Objective】The underdeveloped root system of Phoebe sheareri seedlings seriously restricts the reproduction of P.sheareri seedlings,and exogenous hormones can regulate the morphological development of plant roots.P.sheareri seedlings were treated with paclobutrazol(PP_(333))and 6-benzylaminopurine(6-BA)to analyze their effects on root morphological characteristics.To determine the most suitable hormone and concentration for root development.【Method】Seedlings germinated from mature P.sheareri seeds collected on the same mother tree were used as experimental materials,and three concentrations(PP_(333) concentrations of 0.6,6,60 mg·L^(-1);The concentrations of 6-BA were 10,50 and 75 mg·L^(-1)).The root scanner was used to count the morphological indexes such as taproot length,taproot diameter,total root length and root surface area of P.sheareri seedlings,and the effects of two exogenous hormones on the root morphology of P.sheareri seedlings were analyzed.【Result】Although PP_(333) treatment would increase the growth of P.sheareri seedlings with the increase of taproot length,the number of second-order lateral roots,and the total length of second-order lateral roots,it would promote the increase of the diameter of the main root and the thickening of the main root diameter.Compared with the CK group,PP_(333) and 6-BA promoted the increase of topological index,and promoted the evolution of P.sheareri root to fishtailshaped branch structure.Through principal component analysis and membership function analysis,it was concluded that under hormone treatment,the concentration was 50 mg·L^(-1)6-BA had the best effect on promoting the root development of P.sheareri.【Conclusion】The results showed that different hormones and different concentrations of the same hormone had different regulatory modes on the growth of taproot and lateral roots of P.sheareri.Select 50 mg·L^(-1)6-BA had the best effect on promoting the growth and development of P.sheareri roots.Select 50 mg·L^(-1)6-BA had the best effect on pr
作者
何功秀
宋艳艳
徐培月
胡孔飞
周瑾瑾
李晶
及利
陈军
HE Gongxiu;SONG Yanyan;XU Peiyue;HU Kongfei;ZHOU Jinjin;LI Jing;JI Li;CHEN Jun(College of Forestry,Central South University of Forestry&Technology,Changsha 410004,Hunan,China;Guizhou Forestry Foreign Cooperation and Industrial Development Center,Guiyang 550009,Guizhou,China;Hunan Jinghui Agriculture and Forestry Ecological Technology Co.,Ltd,Changsha 410004,Hunan,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2024年第10期27-35,共9页
Journal of Central South University of Forestry & Technology
基金
国家自然科学基金项目(32071752)
长沙市雨花区科技计划项目(YHKJ-2022-ZN-02)。
关键词
多效唑
6-苄基腺嘌呤
紫楠
根系形态
拓扑指数
paclobutrazol
6-benzylaminopurine
Phoebe sheareri
root morphology
topological indices
