摘要
背景与目的宣威肺癌近年来发病率持续增加,还具有全年龄段高发、女性肺癌死亡率高等特点。因此寻找更多稳定的生物标志物用于宣威肺癌的诊断具有巨大的临床应用前景。本研究旨在探究4个微小RNA(microRNA,miRNA)各自及联合传统肺癌肿瘤标志物在宣威肺癌检测和诊断中的临床应用价值。方法通过检测4个miRNA和5种传统肺癌肿瘤标志物在45例宣威肺癌患者和健康体检者血清中的表达量,采用逻辑回归模型综合评估4个miRNA在宣威肺癌诊断中的灵敏度、特异度和诊断效能等相关统计学指标。结果单个血清miRNA中miR-4646-5P、miR-3654在宣威肺癌组中的相对表达水平与对照组存在显著差异(P<0.05),miR-3654用于诊断的效果最好,灵敏度、特异度和曲线下面积分别为86.7%、82.2%、0.847。miR-3654联合miR-4646-5p、癌胚抗原(carcinoembryonic antigen,CEA)诊断宣威肺癌的效能最高,曲线下面积为0.901,灵敏度为73.3%,特异度为93.3%,阳性预测值为91.7%。结论血清miR-3654在4个miRNA中对宣威肺癌诊断效果最好,并且联合miR-4646-5p、CEA能有效提高其对宣威肺癌的诊断价值,有望作为有价值的宣威肺癌筛查指标。
Background and objective The incidence rate of lung cancer in Xuanwei has been continuously increasing in recent years,and it also features high incidence across all age groups and high mortality rates among female lung cancer patients.Therefore,the search for more stable biomarkers for the diagnosis of Xuanwei lung cancer holds tremendous clinical application prospects.This study aims to explore the clinical application value of these four microRNAs(miRNAs)individually and in combination with traditional lung cancer tumor markers in the detection and diagnosis of Xuanwei lung cancer.Methods By detecting the expression levels of four miRNAs and five traditional lung cancer tumor markers in the serum of 45 Xuanwei lung cancer patients and healthy physical examination participants,the Logistic regression model was employed to comprehensively evaluate the sensitivity,specificity,diagnostic efficacy,and other relevant statistical indicators of the four miRNAs in the diagnosis of Xuanwei lung cancer.Results Among the individual miRNAs,miR-4646-5p and miR-3654 showed significant differences in expression levels between the Xuanwei lung cancer group and the control group(P<0.05).miR-3654 demonstrated the best diagnostic performance with a sensitivity of 86.7%,specificity of 82.2%,and an area under the curve of 0.847.Combining miR-3654 with miR-4646-5p and carcinoembryonic antigen(CEA)resulted in the highest diagnostic efficacy for Xuanwei lung cancer,with a sensitivity of 73.3%,specificity of 93.3%,area under the curve of 0.901,and a positive predictive value of 91.7%.Conclusion Among the four miRNAs,serum miR-3654 exhibits the best diagnostic efficacy for Xuanwei lung cancer.Combined with miR-4646-5p and CEA,its diagnostic value for Xuanwei lung cancer can be effectively enhanced,making it a promising screening indicator for Xuanwei lung cancer.
作者
张仁宁
万鑫蕊
黄萱
李明平
许凯
方绕红
李娅
Renning ZHANG;Xinrui WAN;Xuan HUANG;Mingping LI;Kai XU;Raohong FANG;Ya LI(Department of Clinical Laboratory Medicine,Yunnan Diannan Central Hospital(The First People's Hospital of Honghezhou),Mengzi 661100,China;Department of Clinical Laboratory Medicine,The Third People's Hospital of Kunming,Kunming 650301,China;Department of Clinical Laboratory Medicine,The First Affiliated Hospital of Kunming Medical University,Kunming 650032,China;Yunnan Key Laboratory of Laboratory Medicine,Kunming 650032,China;Yunnan Province Clinical Research Center for Laboratory Medicine,Kunming 650032,China)
出处
《中国肺癌杂志》
CAS
CSCD
北大核心
2024年第9期654-664,共11页
Chinese Journal of Lung Cancer
基金
国家自然科学基金项目(No.82160696)资助国家自然科学基金项目(No.82160696)资助。
关键词
微小RNA
宣威肺腺癌
肺肿瘤
肿瘤标志物
诊断效能
microRNA
Xuanwei lung adenocarcinoma
Lung neoplasms
Tumor markers
Diagnostic efficacy
