摘要
为了挖掘优异西葫芦(Cucurbita pepo L.)种质资源,提高外观品质优的嫩瓜选择效率,本研究利用色差仪对本单位创制的54份西葫芦自交系进行不同嫩瓜皮色分类,并以其中7份(重点4份)代表性材料为研究对象,对西葫芦嫩瓜皮叶绿素合成代谢与其皮色形成的关联性进行分析。结果表明,叶绿素是决定偏白色、浅绿色、翠(青)绿色、绿色和深绿色西葫芦嫩瓜皮色的主要色素。其中,叶绿素a含量占总含量的49.20%~60.58%,是决定嫩瓜皮色深浅的主要因素。颜色最鲜艳且更加偏绿色的翠(青)绿色嫩瓜皮材料,其色度值(C)显著大于其他皮色,而叶绿素a/叶绿素b和红绿值(a*)分别显著大于和小于偏白色、浅绿色和深绿色皮色。深绿色、翠(青)绿色和偏白色嫩瓜皮叶绿素合成存在胆色素原转化为尿卟啉原Ⅲ和粪卟啉原Ⅲ转化为原卟啉IX两个受阻点,且后一转换阶段是导致偏白色和翠(青)绿色嫩瓜皮叶绿素合成受阻、叶绿素含量骤降的主要原因。叶绿素合成减弱与叶绿素代谢中的叶绿素酶降解活性增强以及抗氧化酶中的超氧化物歧化酶和过氧化氢酶活性减弱之间呈显著相关,这也证明了嫩瓜皮偏白色的成因。超氧化物歧化酶和过氧化氢酶活性与嫩瓜皮叶绿素含量呈显著相关,这两种酶延缓了翠(青)绿色和深绿色嫩瓜皮叶绿素含量降低速率。本研究结果为深入解析不同嫩瓜皮色,特别是翠(青)绿色嫩瓜皮呈色机理以及挖掘、利用相关优异特异资源提供了理论依据和技术支撑。
In order to explore the excellent germplasm resources of Cucurbita pepo L.and improve the selection efficiency of tender fruit appearance quality,this study classified the skin color of tender fruit using colorimeter in 54 self-bred lines of zucchini developed by our unit.Based on seven representative lines(including four key lines),the correlation between chlorophyll synthesis metabolism and its skin color formation was analyzed.The results showed that chlorophyll was the main pigment that determined the skin color such as partial white,light green,emerald(vivid)green,green and dark green.The chlorophyll a accounted for 49.20%to 60.58%of the total chlorophyll content,as the main determinant for the deep and light skin color.The emerald(vivid)green skin with the most vibrant and greenish color had significantly higher chromaticity value(C)than other skin color,while chlorophyll a/chlorophyll b and the red green value(a*)were significantly higher and lower respectively than those of partial white,light green,and dark green.The synthesis of chlorophyll in lines with dark green,emerald(vivid)green,and partial white maintained two bottlenecks:PBG to Urogen Ⅲ,and Coprogen Ⅲ to Proto-IX.However,the latter transition stage was the main reason in the chlorophyll synthesis blocked,and sudden decrease of chlorophyll content of partial white and emerald(vivid)green.There was a significant correlation between chlorophyll synthesis weaken and chlase in chlorophyll metabolism enzyme activity enhance degradation,superoxide dismutase(SOD)and catalase(CAT)activities weaken,those also the reason of partial white skin color.SOD and CAT activities in antioxidant enzymes were significantly correlated with the chlorophyll content of the skin of tender fruit,thereby SOD and CAT delaying the rate of chlorophyll reduction in emerald(vivid)green and dark green.This study provided theoretical basis and technical support for in-depth analysis of different skin color of tender fruit especially the color formation mechanism of emerald(viv
作者
李可悦
王丹丹
刘江涛
申琼
盖少杰
武峻新
LI Keyue;WANG Dandan;LIU Jiangtao;SHEN Qiong;GAI Shaojie;WU Junxin(College of Horticulture,Shanxi Agricultural University,Taiyuan 030031)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2024年第8期1336-1346,共11页
Journal of Plant Genetic Resources
基金
山西农业大学生物育种工程(YZGC118)
山西省现代农业产业技术体系建设专项(2023CYJSTX08-02)。
关键词
西葫芦
嫩瓜皮色
翠(青)绿色
叶绿素
合成代谢
zucchini(Cucurbita pepo L.)
the skin color of tender fruit
emerald(vivid)green
chlorophyll
synthesis metabolism
