摘要
为发掘棉花黄萎病生防资源,探究黏细菌在棉花黄萎病生物防治中的应用潜力。本研究以前期实验室分离、保存的黏细菌菌株为材料,采用对峙培养法、混合培养法和对扣培养法等测定黏细菌对大丽轮枝菌的抗菌活性,从中筛选出对大丽轮枝菌具有较好拮抗作用的菌株。温室条件下测定黏细菌发酵液和固体菌剂对棉花黄萎病的盆栽防效以及对棉花生长的影响,并利用形态学和分子生物学方法对生防潜力黏细菌菌株进行鉴定。结果显示,筛选出的4株黏细菌不仅对大丽轮枝菌菌丝具有较好的捕食功效并能够抑制孢子的萌发,其产生的胞外代谢产物也表现出一定的抑菌活性,具有多重抗菌机制。盆栽试验发现,4株黏细菌的发酵液对棉花黄萎病的防效仅为11.56%~38.23%,而以白星花金龟虫砂制备固体菌剂防效均较高,KS01、NSE37、NSE41和KY01对棉花黄萎病的防效分别为79.83%、77.02%、66.57%和58.99%。经形态学和分子生物学鉴定,菌株KS01和KY01为Myxococcus sp.,NSE41和NSE37为Cystobacter sp.。表明4株黏细菌对棉花黄萎病具有生物防治的应用潜力。
To explore biocontrol resources for cotton Verticillium wilt and evaluate the potential application of myxobacteria in managing this disease,we utilized antagonistic activity of mycobacterial strains previously isolated and preserved in our laboratory against Verticillium dahlia were assessed using confrontation culture,mixed culture and dual culture methods,and strains with strong antagonistic effects selected.The efficacy of mycobacterial fermentation broth and solid bacterial formulations against cotton Verticillium wilt was evaluated under greenhouse conditions,along with their impact on cotton growth.Morphological and molecular biological techniques were employed to identify the biocontrol potential of the selected mycobacterial strains.The results demonstrated that the four selected mycobacterial strains effectively preyed on V.dahliae hyphae,inhibited spore germination,and exhibited antibacterial activities through multiple mechanisms.Pot experiments revealed that the fermentation broth of these strains had control effects on cotton Verticillium wilt,with the efficacies ranging from 11.56%to 38.23%.However,efficacies of solid bacterial formulations of strains KS01,NSE37,NSE41 and KY01 on cotton Verticillium wilt were 79.83%,77.02%,66.57%and 58.99%,respectively.Based on morphological and molecular analyses,the strains KS01 and KY01 were identified as Myxococcus sp.,and strains NSE41 and NSE37 as Cystobacter sp..The above results showed that four mycobacterial strains had biocontrol potential against cotton Verticillium wilt.
作者
潘雯
窦新玉
罗明
马德英
路伟
韩剑
PAN Wen;DOU Xinyu;LUO Ming;MA Deying;LU Wei;HAN Jian(Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region/Engineering Research Centre of Cotton,Ministry of Education/College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《中国生物防治学报》
CSCD
北大核心
2024年第5期1074-1087,共14页
Chinese Journal of Biological Control
基金
国家重点研发计划(2022YFD1400304)
新疆维吾尔自治区重点研发专项(2022B02033-1)
新疆棉花产业技术体系(XJARS-03)。
关键词
黏细菌
棉花黄萎病
捕食
筛选
生物防治
myxobacterium
cotton Verticillium wilt
predation
screening
biological control
