摘要
波吉卵囊藻(Oocystis borgei)是一种能够维持水体稳定的有益绿藻。本研究采取混合培养法,探究其与有害蓝藻铜绿微囊藻(Microcystis aeruginosa)之间的竞争关系,同时采用滤液培养的方法研究波吉卵囊藻对铜绿微囊藻的化感作用。结果表明:波吉卵囊藻能有效抑制铜绿微囊藻的生长(p<0.05),而铜绿微囊藻对波吉卵囊藻的抑制效果较差。在波吉卵囊藻滤液胁迫下,铜绿微囊藻生长受到显著抑制(p<0.05),叶绿素a停止积累,其SOD活性先上升再下降,MDA含量持续增加,透射电镜结果显示,细胞超微结构完全损坏,可以推断,波吉卵囊藻胁迫下使铜绿微囊藻产生过量的氧自由基,破坏其细胞膜、抗氧化酶系统和光合系统,从而抑制其生长。
Oocystis borgei is a benefic green alga that can maintain water stability.In this study,a coculture method was used to explore the competition between O.borgei and Microcystis aeruginosa,a harmful cyanobacteria,and a filtrate culture method was used to study the allelopathy of O.borgei on M.aeruginosa.The results showed that O.bogei effectively inhibits the growth of M.aeruginosa(p<0.05)while M.aeruginosa had a lesser inhibitory effect on O.bogei than O.bogei on M.aeruginosa.Under the stress of O.bogei filtrate,the growth of M.aeruginosa was significantly inhibited(p<0.05)and its chlorophyll a content stopped to accumulate simultaneously with its SOD activity which increased first and decreased then,and its MDA content which continuously increased.In addition,its cellular ultrastructure was completely destroyed as was observed under transmission electron microscope.From our findings,it can be inferred that O.bogei induces M.aeruginosa to produce excessive oxygen free radicals,destroys its cell membrane and antioxidant enzyme and photosynthetic systems,thus inhibits its growth finally.
作者
王孝谦
黄翔鹄
李长玲
张玉蕾
罗国玲
王新宇
张宁
WANG Xiaoqian;HUANG Xianghu;LI Changling;ZHANG Yulei;LUO Guoling;WANG Xinyu;ZHANG Ning(College of Fisheries of Guangdong Ocean University,Zhanjiang 524025,China;Engineering Technology Research Center for Algae Breeding and Application of Guangdong Province,Zhanjiang 524025,China)
出处
《海洋湖沼通报》
CSCD
北大核心
2023年第3期48-55,共8页
Transactions of Oceanology and Limnology
基金
深圳市大鹏新区产业发展专项基金项目(KY20180112)
广东省普通高校青年创新人才项目(2018KQNCX102,2018KQNCX110)。
关键词
化感作用
铜绿微囊藻
波吉卵囊藻
抗氧化酶
allelopathic effect
Microcystis aeruginosa
Oocystis borgei
antioxidant enzyme
