摘要
为探明湖北省地方杏品种资源的遗传多样性,以湖北省24个地方杏品种为试验材料,采用SSR分子标记结合荧光毛细管电泳技术开展研究。结果表明,来自杏、桃、扁桃和樱桃上的14对引物共扩增出162个多态性等位基因位点,平均每个位点的等位基因数目为11.571。有效等位基因总数为90.312,平均每个位点的有效等位基因数目为6.451。观测杂合度(Ho)、期望杂合度(He)、香农指数(I)的平均值分别为0.652、0.813、2.031,多态性信息含量(PIC)平均值为0.794,说明所选近源物种的引物具有较高的通用性,多态性丰富。在相似系数0.580处将24个品种分为3个大组,第1组由洪平杏独立成组,第2组由榔梅、杏梅等4个品种组成,第3组由米杏、五祖杏等19个品种组成。可见,湖北省地方杏品种具有丰富的遗传多样性,采用SSR特异性引物可以鉴别湖北省地方杏品种。
To explore the genetic diversity of local apricot variety resources in Hubei Province,24 local apricot varieties materials were taken as test materials,and SSR molecular markers combined with fluorescence capillary electrophoresis technology were used for the study.The results showed that 14 pairs of primers from apricots,peaches,almonds,and cherries amplified a total of 162 polymorphic effective allele loci,with an average of 11.571 alleles per locus.The total number of effective alleles was 90.312,with an average of 6.451 effective alleles per locus.The average values of observed heterozygosity(Ho),expected heterozygosity(He),and Shannon's information index(I)were 0.652,0.813,and 2.031,respectively.The average value of polymorphic information content(PIC)was 0.794,indicating that the primers for the selected related species had high universality and rich polymorphism.At a similarity coefficient of 0.580,24 varieties were divided into three large groups.The first group was independently composed of Hongping apricot,the second group consisted of four varieties including Langmei and Xingmei et al,and the third group consisted of 19 varieties including rice apricot and Wuzu apricot.It could be seen that local apricot varieties in Hubei Province had rich genetic diversity,and SSR specific primers could effectively identify Hubei local apricot varieties.
作者
叶缘铭
蔡化
田瑞
董志丹
张豫
蒋迎春
吴黎明
何利刚
刘模发
YE Yuan-ming;CAI Hua;TIAN Rui;DONG Zhi-dan;ZHANG Yu;JIANG Ying-chun;WU Li-ming;HE Li-gang;LIU Mo-fa(Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences/Pomology and Tea Sub-center of Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees,Wuhan 430064,China)
出处
《湖北农业科学》
2024年第10期97-101,共5页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目(2021-620-000-001-01)
湖北省种业高质量发展专项资金(HBZY2023A001-52)
湖北省农业科技重点课题与平台专项(2021-620-005-003)。
关键词
地方杏
种质资源
荧光SSR标记
遗传多样性
湖北省
local apricot
germplasm resources
fluorescent labeled SSR marker
genetic diversity
Hubei Province
