摘要
目的研究Nectin-4在浸润性膀胱尿路上皮癌(BUC)组织中的表达及其临床意义,为BUC的临床诊断提供参考。方法应用免疫组织化学染色(IHC)法及RNAscope技术检测60例浸润性BUC及40例膀胱黏膜慢性炎组织中Nectin-4的表达,分析对比两种方法的检测结果,并探讨其与浸润性BUC临床病理特征的关系。在此基础上进一步分析BUC组织中Nectin-4蛋白表达与人类表皮生长因子受体2(Her-2)及程序性死亡因子配体1(PD-L1)的相关性。结果IHC法检测Nectin-4蛋白在浸润性BUC组及炎症组中的阳性表达率分别为78.33%(47/60)、17.50%(7/40);RNAscope技术检测Nectin-4 mRNA在浸润性BUC组及炎症组中表达的阳性表达率分别为83.33%(50/60)、12.50%(5/40);两种方法检测浸润性BUC组及炎症组中Nectin-4表达的Kappa值分别为0.732及0.610,一致性一般;Nectin-4蛋白在浸润性BUC中的表达与肌层浸润、组织学分级、脉管癌栓、淋巴结转移、临床分期相关(P<0.05);Nectin-4 mRNA在浸润性BUC中的表达与肿瘤最大径、肌层浸润、组织学分级、脉管癌栓、淋巴结转移、临床分期相关(P<0.05);Nectin-4在浸润性BUC中的高表达和Her-2表达呈正相关(P=0.002),与PD-L1表达无相关性(P>0.05)。结论Nectin-4在浸润性BUC中高表达,且通常和不良预后的病理参数相关,检测浸润性BUC中Nectin-4表达将有助于指导其临床的诊治。
Objective To explore the expression of Nectin-4 in invasive bladder urothelial carcinoma(BUC)tissue and its clinical significance,so as to provide reference for clinical diagnosis and treatment of BUC.Methods Nectin-4 expression in 60 cases of invasive BUC and 40 cases of chronic inflammation of bladder mucosa was detected with immunohistochemical staining(IHC)and RNAscope.The results of the two methods were analyzed and compared,and the relationship between the two methods and the clinicopathological characteristics of invasive BUC was discussed.The correlation between the protein expression of Nectin-4 in BUC tissues,human epidermal growth factor receptor 2(Her-2)and programmed death factor ligand 1(PD-L1)was analyzed.Results The positive protein expression rates of Nectin-4 detected by IHC were 78.33%(47/60)and 17.50%(7/40)in the invasive BUC group and inflammatory group,respectively,while the positive mRNA expression rates of Nectin-4 detected by RNAscope were 83.33%(50/60)and 12.50%(5/40),respectively.The Kappa values of Nectin-4 in the invasive BUC group and inflammatory group were 0.732 and 0.610,respectively,with general consistency.The protein expression of Nectin-4 in invasive BUC was correlated with muscular invasion,histological grade,vascular thrombus,lymph node metastasis and clinical stage(P<0.05).The mRNA expression of Nectin-4 in invasive BUC was correlated with max tumor diameter,muscular invasion,histological grade,vascular thrombus,lymph node metastasis and clinical stage(P<0.05).The high expression of Nectin-4 in invasive BUC was positively correlated with the expression of Her-2(P=0.002),but not with the expression of PD-L1(P>0.05).Conclusion Nectin-4 is highly expressed in invasive BUC,and is usually associated with the pathological parameters of poor prognosis.Detection of Nectin-4 expression will help to guide clinical diagnosis and treatment.
作者
宋慧茹
罗丹
温俊秀
倪璐
张可心
王琦
杨柳
宋旭东
董丽儒
SONG Huiru;LUO Dan;WEN Junxiu;NI Lu;ZHANG Kexin;WANG Qi;YANG Liu;SONG Xudong;DONG Liru(Department of Pathology,The Affiliated Hospital of North China University of Science and Technology,Tangshan 063000;Department of Pathology,School of Basic Medicine Sciences,North China University of Science and Technology,Tangshan 063000;Department of Pathology,The Second Hospital of Shenzhen,Shenzhen 518035,China)
出处
《现代泌尿外科杂志》
CAS
2024年第10期903-908,共6页
Journal of Modern Urology
基金
2020年度河北省医学适用技术跟踪项目(No.GZ2020089)。
