摘要
目的:通过蒙医温针对大鼠睡眠剥夺的干预研究来探究蒙医温针对失眠及其导致的氧化应激的影响并分析相关机制。方法:本研究采用Wistar大鼠作为实验动物,随机分为空白组、模型组、蒙医温针组和安定组,利用腹腔注射PCPA模拟睡眠剥夺动物模型,描记入组大鼠体质量变化及神经细胞改变。检测大鼠脑中5-HIAA、5-HT与NE含量以及脑干中SOD和NO含量。明确睡眠剥夺大鼠脑干组织CCK-8、GABA变化。结果:模型组大鼠脑干网状结构中5-HIAA、5-HT和CCK-8水平低于空白组,差异有统计学意义(P<0.01),而NE、SOD和NO含量显著增加,差异有统计学意义(P<0.01)。经睡眠剥夺后,模型组大鼠脑干细胞中内粗面内质网减少,突触存在融合现象。此外,与模型组比较,蒙医温针组的5-HT、5-HITT含量升高和NE含量降低,差异有统计学意义(P<0.01);另外,与模型组和蒙医温针组比较,安定组的NE含量均显著降低,差异有统计学意义(P<0.01),蒙医温针组和安定组大鼠脑干网状组织内的CCK-8和GABA水平高于模型组,差异有统计学意义(P<0.05或P<0.01)。结论:蒙医温针对神经细胞具有保护作用,恢复5-HT与NE平衡,恢复睡眠-觉醒节律,并可缓解因睡眠剥夺导致氧化应激而致的能量消耗,改善进食增多、体质量减轻状态。
Objective:To explore the effect and mechanism of Mongolian warm needling on insomnia and induced oxidative stress response by the experimental study of Mongolian warm needling intervention on sleep deprivation in rats.Methods:The Wistar rats were randomly divided into the blank group,the model group,the Mongolian warm needling group,and the Diazepam group.The sleep deprivation rat model was established through the administration of PCPA via intraperitoneal injection.The alterations in the body weight of rats after sleep deprivation were observed,and the changes in rat nerve cells were observed by electron microscopic.The changes in 5-HIAA,5-HT and NE in brain of rats were observed by fluorescence spectrophotometer.The changes in the contents of SOD and NO in brain stem were observed by grating spectrophotometer.The expressions of CCK-8 and GABA in the brain stem of sleep deprived rats was observed by immunohistochemical staining.Results:The contents of 5-HIAA,5-HT and CCK-8 in reticular formation of the brainstem were decreased,whereas the contents of NE,SOD and NO were increased in the model group(P<0.01).After sleep deprivation,brain stem cells in the model group of rats exhibited blurred cell membrane boundaries,cloudy cell structures,and vacuolar degeneration.Additionally,the miotchondria showed vacuolar degeneration and significant swelling.Furthermore,the content of rough endoplasmic reticulum within the cells decreased,and synapses fused.However,there were no significant changes in the Mongolian warm needling group.Compared with those in the model group,the contents of 5-HT and 5-HITT were increased,whereas the content of NE was decreased in the Mongolian warm needling group(P<0.01).The content of NE was significantly decreased in the Diazepam group compared with that in the model group and the Mongolian warm needling group(P<0.01).Immunohistochemical staining confirmed that after sleep deprivation,the contents of CCK-8 and GABA in reticular formation of the brainstem were significantly increased in the Mo
作者
阿茹娜
阿古拉
包青林
木日根吉雅
斯楞格
Aruna;Agula;BAO Qinglin;Murigen Jiya;Slinge(Inner Mongolia Medical University,Hohhot 010110,China;Inner Mongolia University of Science and Technology,Baotou 014040,China;Inner Mongolia Autonomous Region International Mongolian Medical Hospital,Hohhot 010020,China)
出处
《针灸临床杂志》
2024年第10期78-83,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金,编号:82074577、82160945
内蒙古医科大学蒙医学“一流学科”建设项目,编号:myxylxk2022023、myxylxk2022025
内蒙古医科大学蒙药学“一流学科”青年教师科研创新项目,编号:MYX2023-S01
内蒙古医科大学面上项目,编号:YKD2021MS049
内蒙古自治区蒙医药协同创新中心科研项目,编号:MYYXTPY202207
内蒙古自治区卫健委医疗卫生科技计划项目,编号:202201212。
关键词
蒙医温针
睡眠剥夺
氧化应激
Mongolian warm needling
Sleep deprivation
Oxidative stress
