摘要
目的建立天麻健脑颗粒(TJG)的指纹图谱及测定含量的方法,结合化学计量学分析综合评价TJG的质量。方法采用高效液相色谱(HPLC)法建立13批(S1~S13)TJG的指纹图谱,测定肌苷、天麻素、巴利森苷B、巴利森苷E的含量;采用SPSS 20.0和SIMCA 18软件进行聚类分析、主成分分析和正交偏最小二乘法-判别分析,以变量重要性投影(VIP)值大于1为标准,筛选影响质量的标志性成分。结果13批TJG共标定28个共有峰,其相似度均大于0.9;共指认了7个共有峰,分别为天麻素、对羟基苯甲醇、巴利森苷B、巴利森苷E、钩藤碱、肌苷、红景天苷。13批TJG聚为3类,其中S1~S2、S8~S10、S12为一类,S3、S7为一类,S4~S6、S11、S13为一类;肌苷的VIP值大于1。肌苷、天麻素、巴利森苷B、巴利森苷E的含量分别为62.637~176.677、17.821~37.642、5.748~16.077、5.660~13.510μg/g。结论所建HPLC指纹图谱及含量测定方法稳定可靠、重复性高,结合化学计量学分析可用于评价TJG的质量;肌苷可能是影响TJG质量的标志性成分;13批TJG质量存在差异。
OBJECTIVE To establish the fingerprints of Tianma jiannao granules(TJG)and the method for content determination to evaluate the quality of TJG comprehensively combined with chemometric analysis.METHODS High-performance liquid chromatography(HPLC)was used to establish the fingerprints of 13 batches(S1-S3)of TJG and determine the contents of inosine,gastrodin,parishin B and parishin E.Cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis were performed using SPSS 20.0 and SIMCA 18 software;using variable importance projection(VIP)value greater than 1 as a criterion,marker components that affected quality were screened.RESULTS A total of 28 common peaks were identified in the 13 batches of TJG with similarities greater than 0.9,and 7 common peaks were identified,which were gastrodin,p-hydroxybenzyl alcohol,parishin B,parishin E,rhynchophylline,inosine and salidroside.The 13 batches of TJG were clustered into 3 categories,S1-S2,S8-S10 and S12 were clustered into one category;S3 and S7 were clustered into one category;S4-S6,S11 and S13 were clustered into one category.VIP of inosine was greater than 1.The contents of inosine,gastrodin,parishin B and parishin E were 62.637-176.677,17.821-37.642,5.748-16.077 and 5.660-13.510μg/g.CONCLUSIONS The established HPLC fingerprints and content determination method are stable,reliable and highly reproducible,which can be used to evaluate the quality of TJG in combination with chemometric analysis.Inosine may be a marker component that affects the quality of TJG.There are differences in the quality of 13 batches of TJG.
作者
杜锦顔
莫静媛
谢询
黄小玲
伍小玲
王利胜
DU Jinyan;MO Jingyuan;XIE Xun;HUANG Xiaoling;WU Xiaoling;WANG Lisheng(College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Dept.Three of Internal Medicine,Dongguan Hospital of Traditional Chinese Medicine,Guangdong Dongguan 523000,China)
出处
《中国药房》
CAS
北大核心
2024年第20期2482-2487,共6页
China Pharmacy
基金
广东省普通高校重点领域专项
东莞市高水平医院建设专项
东莞市社会发展科技项目(No.20231800903722)。
关键词
天麻健脑颗粒
指纹图谱
含量测定
聚类分析
主成分分析
正交偏最小二乘法判别分析
Tianma jiannao granules
fingerprint
content determination
cluster analysis
principal component analysis
orthogonal partial least squares-discriminant analysis
