摘要
目的:探讨不同剂量阿里红多糖(Fomes officinalis Ames polysaccharides,FOP)对运动性免疫抑制(Exercise-induced immunosuppression,EIS)大鼠肠道菌群及免疫功能的保护作用。方法:50只7~8周龄SPF级雄性SD大鼠随机分为对照组(NC组)、运动训练组(Ex组)、运动训练+低剂量FOP组(LFOP组,40 mg/(kg·d))、运动训练+中剂量FOP组(MFOP组,60 mg/(kg·d))和运动训练+高剂量FOP组(HFOP组,80 mg/(kg·d))。Ex组和各剂量FOP组采用6周跑台训练构建EIS模型,LFOP、MFOP、HFOP组每次训练后灌胃FOP。干预结束后,酶联免疫吸附试验检测血清免疫球蛋白G(Immunoglobulin G,IgG)、免疫球蛋白M(Immunoglobulin M,IgM)、白介素6(Interleukin-6,IL-6)、白介素10(Interleukin-10,IL-10)、干扰素γ(Interferon-γ,INF-γ)、肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)、脂多糖(Lipopolysaccharide,LPS)水平及肠道短链脂肪酸含量;细胞分析仪检测血清CD4+、CD8+的数量;16S rDNA检测大鼠肠道菌群结构变化;Western blot检测结肠组织紧密连接蛋白(Zonula occludens-1,ZO-1)、封闭蛋白(Occludin)、紧密连接蛋白4(Claudin4)蛋白表达。结果:与Ex组比较,LFOP、MFOP、HFOP组大鼠血清IgG、IgM、INF-γ、TNF-α、CD4^(+)和CD8^(+)极显著上升(P<0.01),血清IL-6、IL-10和LPS水平极显著降低(P<0.01);肠道短链脂肪酸含量和拟杆菌门、疣微菌门及乳杆菌属、拟杆菌属、毛螺菌属(未分类)、艾克曼菌属相对丰度极显著上升(P<0.01);结肠组织ZO-1、Occludin、Claudin4蛋白表达极显著上调(P<0.01)。此外,MFOP、HFOP组大鼠脾脏指数、胸腺指数、肠道菌群α多样性较Ex组极显著上升(P<0.01)。结论:FOP可能通过减轻炎症改善肠道菌群结构及活性,提高机体免疫功能,进而抑制EIS的发生发展。
Objective:To investigate the protective effects of different doses of Fomes officinalis Ames polysaccharides (FOP) on intestinal flora and immune function in exercise-induced immunosuppression (EIS) rats. Methods: Fifty SPF male SD rats aged 7 to 8 weeks were randomly divided into control group (NC group), exercise training group (Ex group), exercise training+low-dose FOP group (LFOP group, 40 mg/(kg·d)), exercise training+medium-dose FOP group (MFOP group, 60 mg/(kg·d)) and exercise training+high-dose FOP group (HFOP group, 80 mg/(kg·d)). Ex group and FOP group were trained on the treadmill for 6 weeks to construct EIS model. LFOP, MFOP and HFOP groups were given FOP after each training. After the intervention, serum IgG, IgM, IL-6, IL-10, INF-γ, TNF-α, LPS levels and intestinal short-chain fatty acid content were detected by enzyme-linked immunosorbent assay. The number of serum CD4+ and CD8+ was detected by cell analyzer. 16S rDNA was used to detect the structural changes of intestinal flora. Western blot was used to detect the protein expression of ZO-1, Occludin, Claudin4 in the colon tissue. Results: Compared with Ex group, serum IgG, IgM, INF-γ, TNF-α, CD4^(+) and CD8^(+) of rats in LFOP, MFOP and HFOP groups were significantly increased (P<0.01), while serum levels of IL-6, IL-10 and LPS were significantly decreased (P<0.01). Intestinal short-chain fatty acid content and the relative abundance of Bacteroidetes, Verrucobacteria and Lactobacillus, Bacteroidetes, Spirillum (not classified) and Ekmanella were significantly increased (P<0.01). The expression of ZO-1, Occludin, Claudin4 proteins in colon tissue was significantly up-regulated (P<0.01). In addition, spleen index, thymus index, intestinal flora alpha diversity in MFOP and HFOP groups were significantly increased compared with Ex group (P<0.01). Conclusion: FOP may improved the structure and activity of intestinal flora by reducing inflammation, enhanced the body's immune function, and then inhibited the development of EIS.
作者
孔海军
张亮
谌晓安
KONG Haijun;ZHANG Liang;CHEN Xiaoan(College of Physical Education,Kashi Unversity,Kashi 844000,China;College of Physical Education,Tibet University for Nationalities,Xianyang 712082,China;College of Physical Education Science,Jishou University,Xiangxi 416000,China)
出处
《食品工业科技》
CAS
北大核心
2024年第21期358-367,共10页
Science and Technology of Food Industry
基金
新疆维吾尔自治区喀什地区科技计划(20200024)
新疆维吾尔自治区高校科技计划(XJEDU2021SY040)。
关键词
运动性免疫抑制
肠道菌群
免疫功能
阿里红多糖
exercise-induced immunosuppression
intestinal flora
immune function
Fomes officinalis Ames polysa-ccharides
