摘要
目的 观察灵芝多糖口服液对小鼠免疫功能的影响。方法 ①制备S180 瘤小鼠模型 ;②皮下注射环磷酰胺 (cytoxan ,CY) 5 0mg·kg·d-1,制备免疫抑制鼠模型。灵芝多糖口服液 15 0mg·kg·d-1、30 0mg·kg·d-1经灌胃给药 ,连续 10d ,分别观察抑瘤效应 ;用MTT方法检测NK细胞杀伤活性 ;对CY免疫抑制小鼠 ,检测腹腔Mφ细胞吞噬功能、脾细胞增殖指数 ,计算给药后各组小鼠脾脏和胸腺指数。结果 灵芝多糖口服液 15 0mg·kg-1、30 0mg·kg-1对小鼠S180 瘤有明显的抑制作用 (P <0. 0 1) ,能够对抗CY所致的小鼠脾和胸腺指数减少 ,明显增加免疫系统吞噬功能及脾细胞增殖数 ,灵芝多糖口服液能明显增加NK细胞的杀伤活性。结论 灵芝多糖口服液抑制小鼠S180 瘤生长 ,增强免疫功能。
Objective: To investigate the effects of ganoderma lucidum polysaccharide oral liquid on immunologic function in mice. Methods: ①Producing mouse model bearing S 180 tumor, ②Producing immunodepressive mouse model by injecting subcutaneously cytoxan(CY)50 mg·kg·d -1 . Ten days after having been orally administered with 150 、 300 mg·kg -1 oral liquid, the mice bearing S 180 tumor were killed, the tumor-inhibitory effects were observed. The lethal activity of NK cells was examined with MTT. The phagocytosis of Mφ,proliferation index of splenocyte, spleen index and thymus index of CY-induced immunodepressive mice were determined. Results:150 or 300 mg·kg -1 oral liquid significantly depressed the S 180 tumor (P<0.01 vs. control) . The coefficients of spleen and thymus, the phagocytic function of phagocytes, the coefficient of splenocyte proliferation and the activity of NK cell in immunodepressive mice were obviously increased. Conclusion:Oral liquid has the functions of inhibiting S 180 tumor and strengthening organism's immunity.
出处
《泰山医学院学报》
CAS
2004年第6期586-588,共3页
Journal of Taishan Medical College
关键词
灵芝多糖口服液
免疫功能
抗肿瘤
ganoderma lucidum polysaccharide oral liquid
immunologic function
antineoplastic
