摘要
目的探讨丰富环境对慢性应激所致抑郁样小鼠行为及海马锥体神经元突触传递功能的影响。方法36只7周龄SPF级雄性C57BL/6J小鼠按照随机区组法分为对照组、模型组、丰富环境组,每组12只。模型组和丰富环境组小鼠采用慢性不可预知性温和应激方法建立抑郁模型,造模期间丰富环境组小鼠每天在丰富环境中生活6 h,其余时间与对照组、模型组小鼠均生活在常规环境中。采用糖水偏爱实验、悬尾实验、强迫游泳实验检测小鼠抑郁行为,采用旷场实验检测小鼠运动能力及焦虑行为,采用Morris水迷宫实验检测小鼠认知功能。采用离体电生理技术记录海马场兴奋性突触后电位(field excitatory post-synaptic potentials,fEPSP),采用全细胞记录检测海马锥体神经元自发性兴奋性突触后电流(spontaneous excitatory post-synaptic currents,sEPSC)及微小兴奋性突触后电流(miniature excitatory post-synaptic currents,mEPSC)。采用SPSS 23.0软件进行统计分析,多组间比较采用单因素方差分析和重复测量方差分析。结果(1)行为学结果显示:3组小鼠在旷场中央区的活动总时间、糖水偏爱率、悬尾和强迫游泳不动时间均差异有统计学意义(F=17.12,26.07,41.13,60.18,均P<0.01)。模型组小鼠中央区活动时间、糖水偏爱率均低于对照组(均P<0.01),悬尾不动时间和强迫游泳不动时间均高于对照组(均P<0.01);丰富环境组小鼠中央区活动时间[(56.56±3.47)s]、糖水偏爱率[(71.22±8.37)%]均高于模型组[(52.56±3.47)s,(59.53±8.72)%](均P<0.05),悬尾不动时间[(94.19±10.77)s]和强迫游泳不动时间[(76.98±12.10)s]均低于模型组[(104.58±8.24)s,(111.41±9.56)s](均P<0.05)。(2)空间学习记忆力结果表明,3组小鼠寻找平台潜伏期的组别与时间交互效应显著(F=12.02,P<0.01);模型组小鼠寻找平台的潜伏期均高于对照组和丰富环境组(均P<0.01)。空间探索结果表明,3组小鼠目标象限停留时�
Objective To investigate the effects of enriched environment on the behavior of depressive-like mice induced by chronic stress and the synaptic transmission function of hippocampal pyramidal neurons.Methods Thirty-six 7-week-old SPF grade male C57BL/6J mice were divided into control group,model group and enriched environment group according to the random zone method,with 12 mice in each group.The mice in model group and enriched environment group were subjected to chronic unpredictable mild stress to establish depression models.During the modeling period,the mice in enriched environment group lived in the enriched environment for 6 hours per day,and lived in the routine environment for the rest time just like the mice in control group and model group.The depressive behaviors of mice were detected by sucrose preference test(SPT),forced swimming test(FST)and tail suspension test(TST).The motor function and anxious behavior of mice were detected by open field test(OFT)and the cognitive function was detected by Morris water maze test.In vitro electrophysiological techniques were used to record field excitatory post-synaptic potentials(fEPSP)in the hippocampus,and whole-cell recording was used to record spontaneous excitatory post-synaptic currents(sEPSC)and miniature excitatory post-synaptic currents(mEPSC)in hippocampal pyramidal neurons.Statistical analysis was conducted by SPSS 23.0 software.Multiple group comparisons of data were conducted by one-way ANOVA and repeated measures ANOVA.Results(1)Behavioral results showed statistically significant differences among the three groups in terms of total activity time in the central area of OFT,sugar water preference rate of SPT and immobility time of TST and FST(F=17.12,26.07,41.13,60.18,all P<0.01).The activity time in the central area of OFT and sugar water preference rate of model group were lower than those in the control group(both P<0.01),and the immobility time of TST and FST were higher than those in the control group(both P<0.01).The activity time in the centra
作者
陈进东
朱传安
马增明
夏玉平
Chen Jindong;Zhu Chuan'an;Ma Zengming;Xia Yuping(Clinical Medicine School of Fujian Medical University,Fuzhou 350122,China;Xiamen Xianyue Hospital,Xianyue Hospital Affiliated to Xiamen Medical College,Fujian Psychiatric Center,Fujian Clinical Research Center for Mental Disorders,Xiamen 361012,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2024年第9期769-775,共7页
Chinese Journal of Behavioral Medicine and Brain Science
基金
福建省2022年省级临床重点专科建设项目(闽卫医政函〔2022〕884号)
福建省卫生健康科技计划项目(2021QNB026)
福建省科技计划项目(2022D008)
厦门市医疗卫生指导性项目(3502Z20214ZD1278)。
关键词
丰富环境
慢性应激
抑郁
海马
突触可塑性
兴奋性突触后电位
兴奋性突触后电流
小鼠
Enriched environment
Chronic stress
Depression
Hippocampus
Synaptic plasticity
Excitatory post-synaptic potential
Excitatory post-synaptic current
Mouse
