摘要
目的探讨慢性不可预见性温和性应激(chronic unpredictable mild stress,CUMS)抑郁模型小鼠海马齿状回颗粒细胞突触传递功能的变化并探讨运动对其影响。方法48只7周龄SPF级雄性C57BL/6J小鼠,按照随机区组分为对照组、模型组、运动组、氟西汀组,每组12只。采用CUMS方法制备抑郁动物模型,造模连续21 d。造模期间,运动组小鼠每天给予滚筒运动训练,氟西汀组小鼠在造模期间每日给予氟西汀腹腔注射(10 mg/kg),其余组小鼠腹腔注射等体积0.9%氯化钠溶液。采用糖水偏好实验、悬尾实验、强迫游泳实验检测小鼠抑郁样行为,采用旷场实验检测小鼠焦虑样行为;采用全细胞记录海马齿状回颗粒细胞自发性兴奋性突触后电流(spontaneous excitatory postsynaptic currents,sEPSC)、自发性抑制性突触后电流(spontaneous inhibitory postsynaptic currents,sIPSC)及微小兴奋性突触后电流(miniature excitatory postsynaptic currents,mEPSC)、微小抑制性突触后电流(miniature inhibitory postsynaptic currents,mIPSC)。采用SPSS 23.0对实验数据进行单因素方差分析。结果(1)行为学结果显示:4组小鼠旷场中央区探索时间、活动总距离及糖水偏好率差异有统计学意义(F=37.85,18.41,28.81,均P<0.01),模型组小鼠旷场中央区探索时间[(47.81±3.51)s]、活动总距离[(19.63±1.24)m]及糖水偏好率[(55.63±9.11)%]均低于对照组(均P<0.01);运动组小鼠旷场中央区探索时间[(59.87±3.25)s]、活动总距离[(23.18±1.24)m]及糖水偏好率[(69.03±8.22)%]均高于模型组(均P<0.05),糖水偏好率低于对照组及氟西汀组(均P<0.01)。4组小鼠悬尾实验不动时间与强迫游泳实验不动时间差异有统计学意义(F=113.70,56.97,均P<0.01),模型组小鼠悬尾实验不动时间、强迫游泳实验不动时间均高于对照组(均P<0.01),运动组小鼠悬尾不动时间、强迫游泳实验不动时间均低于模型组(均P<0.05),且高于对照组(均P<0.01)。(2)4组�
Objective To investigate the changes in synaptic transmission function of hippocampal dentate gyrus granule cells in depression model mice induced by chronic unpredictable mild stress(CUMS)and to explore the effect of exercise on it.Methods Totally 48 SPF grade 7-week-old male C57BL/6J mice were randomly compartmentalised into control group(CTRL group),model group(CUMS group),model+exercise group(CUMS+EXE group),and model+fluoxetine group(CUMS+FLX group),with 12 mice in each group.CUMS method was used for preparating the depression model for 21 d.Mice in the CUMS+EXE group were given roller locomotion training and mice in the CUMS+FLX group were given fluoxetine intraperitoneally(10 mg/kg)daily during the modelling period,and the other two groups were administrated intraperitoneally with equal volume of 0.9%NaCl solution.Sucrose preference test(SPT),tail suspension test(TST)and forced swimming test(FST)were used to detect depressive-like behavior,and open field test(OFT)was used to detect anxiety-like behavior.Whole-cell recordings were used to detect spontaneous excitatory postsynaptic currents(sEPSC),spontaneous inhibitory postsynaptic currents(sIPSC),miniature excitatory postsynaptic currents(mEPSC),and miniature inhibitory postsynaptic currents(mIPSC)in hippocampal dentate gyrus granule cells.One-way ANONA was performed to analyze the data by SPSS 23.0.Results (1)There were statistically significant differences among the 4 groups of mice in terms of activity time in the center,total distance of OFT and sugar preference rate(F=37.85,18.41,28.81 all P<0.01).The activity time in the center((47.81±3.51)s)and total distance((19.63±1.24)m)of OFT and sugar preference rate((55.63±9.11)%)in the CUMS group were lower than those in the CTRL group(all P<0.01),while those in the CUMS+EXE group((59.87±3.25)s,(23.18±1.24)m,(69.03±8.22)%respectively)were higher than those in the CUMS group(all P<0.05).The sugar preference rate of the CUMS+EXE group was lower than that in the CTRL group and CTRL+FLX group(both P<0.01).T
作者
夏玉平
朱传安
马增明
陈进东
Xia Yuping;Zhu Chuan'an;Ma Zengming;Chen Jindong(The School of Clinical Medicine,Fujian Medical University,Fuzhou 350122,China;Xiamen Xianyue Hospital,Xianyue Hospital Affiliated with Xiamen Medical College,Fujian Psychiatric Center,Fujian Clinical Research Center for Mental Disorders,Xiamen 361012,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2024年第7期590-596,共7页
Chinese Journal of Behavioral Medicine and Brain Science
基金
福建省卫生健康科技计划项目(2021QNB026)
福建省科技计划项目(2022D008)
厦门市医疗卫生指导性项目(3502Z20214ZD1278)
厦门市仙岳医院重大科研项目(2021-XYZD02)。
关键词
运动
海马
颗粒细胞
兴奋/抑制比值
突触传递
全细胞记录
小鼠
Exercise
Hippocampus
Granule cells
Excitation/inhibition ratio
Synaptic transmission
Whole-cell recording
Mouse
