摘要
【目的】为进一步探明LsABF2参与叶用莴苣抽薹过程中的分子机制,进一步了解ABA通路对叶用莴苣抽薹的影响,为未来抗抽薹叶用莴苣的育种提供理论支持。【方法】使用易抽薹叶用莴苣品种‘GB-30’为试验材料,利用VIGS技术和qRT-PCR技术分析了LsABF2基因在叶用莴苣抽薹中的作用,以及LsABF2与LsARF3、LsARF3L之间的联系。【结果】瞬时沉默LsABF2后,对植株进行株高和茎长的观察测量,经过3次技术重复、3次生物重复,发现沉默LsABF2基因24 d后的植株茎长明显高于对照组,并且具有显著性差异。制作植物茎尖的石蜡切片观察茎尖生长点形态,结果显示空白和阴性对照组植株尚未进入进入生殖生长时期,茎尖生长点位置呈现凸起状态;而试验组植株中茎尖生长点呈扁平状,表明已经开始花芽分化,进入生殖生长时期。因此,使用VIGS技术瞬时沉默LsABF2基因促进了叶用莴苣抽薹。通过荧光定量PCR技术表明LsABF2与LsARF3、LsARF3L有相互作用,LsABF2沉默后二者的表达量升高。【结论】LsABF2基因与叶用莴苣先期抽薹相关,在叶用莴苣抽薹中起抑制作用。并且验证了在叶用莴苣中生长素调节因子LsARF3、LsARF3L与脱落酸调节因子LsABF2之间有相互作用,共同调节叶用莴苣的抽薹。
[Objective]To further explore the molecular mechanism of LsABF2 in the process of leaf lettuce bolting and understand the impact of ABA pathway on leaf lettuce bolting.[Methods]Using the easy to bolt lettuce variety'GB-30'as the experimental material,the role of LsABF2 gene in leaf lettuce bolting was analyzed using virus induced gene silencing(VIGS)technology.[Results]The stem length of LsABF2-silenced plants was significantly higher than that of the controls.The growth points of stem tips in the controls was conical,entering the flower bud differentiation stage in the morning,while in the LsABF2-silenced plants,the growth points of stem tips were flat and had entered the flower bud differentiation stage.Instantaneous silencing of the LsABF2 gene through VIGS technology promote the bolting of lettuce leaves.qRT-PCR showed that LsABF2 negatively regulated the expression levels of LsARF3 and LsARF3L.[Conclusion]The LsABF2 gene is associated with the early bolting of leaf lettuce and plays an inhibitory role in the bolting,and regulates the transcript levels of auxin regulator LsARF3,LsARF3L.
作者
王佳璇
邹小丽
王月英
刘婷婷
韩莹琰
刘超杰
王慧玉
郝敬虹
WANG Jiaxuan;ZOU Xiaoli;WANG Yueying;LIU Tingting;HAN Yingyan;LIU Chaojie;WANG Huiyu;HAO Jinghong(Beijing Key Laboratory of New Technology in Agricultural Application/National Demonstration Center for Experimental Plant Production Education/College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)
出处
《北京农学院学报》
2024年第4期32-37,共6页
Journal of Beijing University of Agriculture
基金
北京市自然科学基金-教委联合重点资助项目(KZ20231002034)
北京市种质创制和品种选育联合攻关项目(G20220628003)
生菜高产优质品种选育及配套栽培技术研究(XY-TD-22-01)。
