摘要
为了探究侏儒型华南鲤(Cyprinus carpio rubrofuscus)生长发育迟缓的分子机制,深入分析血管内皮生长因子(vascular endothelial growth factor, VEGF)在鱼类生长过程中的作用,本研究比较分析了侏儒型华南鲤和生长正常华南鲤(对照组)的生长性状、摄食率和饲料利用率等指标,并扩增和测序分析了华南鲤vegf121基因的cDNA序列,利用实时荧光定量PCR方法(qRT-PCR)检测了生长正常华南鲤vegf121基因的mRNA在11种组织中的表达量,以及在侏儒型鱼的心脏、垂体、大脑、肌肉和下丘脑中的表达水平。摄食实验结果显示,侏儒型华南鲤的摄食率和增重率显著低于体型相似的生长正常华南鲤,而饲料系数与正常鱼无显著差异。同时,获得华南鲤vegf121基因c DNA序列1 235 bp,其中,开放阅读框432 bp,编码144个氨基酸。该蛋白属于VEGF家族的VEGF121亚型。其氨基酸序列与11种已知鱼类VEGF121序列同源性在79.86%以上,而与人类(Homosapiens)和小鼠(Mus musculus)的同源性仅为51.02%和52.74%。VEGF121亚型在人类和鱼类的同源性较低且时空分布存在较大差异,可能与其呼吸方式和呼吸系统的血管形成模式差异有关。qRT-PCR结果显示,在生长正常华南鲤11个组织中均能检测到vegf121mRNA的表达,说明vegf121对于华南鲤不同组织中血管的发育有不同程度的调控作用。比较侏儒型华南鲤和生长正常鱼的下丘脑等5个组织中该基因的表达发现,在侏儒型华南鲤下丘脑组织中的表达水平显著高于生长正常鱼;而在大脑和垂体中,vegf121的表达水平相当;在肌肉和心脏组织中,vegf121的表达水平都较生长正常鱼低,但差异不显著。因此,推测侏儒型华南鲤的生长发育缓慢可能与下丘脑的血管生成异常和器质性病变影响鱼体的食欲调控信号有关,进而导致鱼体生长缓慢。本研究为进一步探讨侏儒型华南鲤生长发育缓慢的原因提供了科学依据。对养殖经济
To explore the molecular mechanism of dwarf fish formation in the cultured population of Cyprinus carpio rubrofuscus and the functions of vascular endothelial growth factor(VEGF)in fish growth,the features of growth trait,feed intake,and feed utilization of dwarf fish were detected and analyzed.Vegf121 cDNA was amplified and sequenced in this study.The expression levels of vegf121 were analyzed by quantitative real time PCR(qRT-PCR)in 11 tissues of normal fish,and in the heart,pituitary,brain,muscle,and hypothalamus of dwarf fish.The results showed that under the culture condition in ponds,the body length distribution of the typical five-month-old C.carpio rubrofuscus population with dwarf individuals showed two obvious independent normal distribution curves.Furthermore,the dwarf individuals formed a small population that distinguished it from the normal and fast-growing fish in terms of body size.The results of the feeding experiment showed that the daily feed intake(DFI)and the weight gain rate(WGR)of fish in the dwarf group were significantly lower than those in the normal group,whereas the feed conversion rate(FCR)showed no significant difference between the two groups.Meanwhile,vegf121 was found to be differentially expressed in C.carpio rubrofuscus.The partial cDNA sequence of vegf121 gene was 1,235 bp with an open reading frame of 432 bp encoding 144 amino acids.According to the predicted amino acid sequence,the protein belongs to the VEGF121 isoform and has a relatively high sequence identity to the VEGF121 proteins of 11 known fish with the lowest identity of 79.86%aligned to Denticeps clupeoides.However,the identities to VEGF121 isoforms to those of Homo sapiens and Mus musculus were lower,at only 51.02%and 52.74%,respectively.The low homology and different spatial and temporal distribution of VEGF121 isoforms between animals and fishes may be related to breathing regulators and the differences in vascularization patterns in the respiratory system.The qRT-PCR results of the expression levels of vegf121
作者
陈烁桢
马冬梅
钟再选
樊佳佳
朱华平
苏换换
CHEN Shuozhen;MA Dongmei;ZHONG Zaixuan;FAN Jiajia;ZHU Huaping;SU Huanhuan(Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture,Guangzhou 510380,China)
出处
《渔业科学进展》
CSCD
北大核心
2024年第6期71-82,共12页
Progress in Fishery Sciences
基金
国家自然科学基金面上项目(32072971)
广东省乡村振兴战略专项资金种业振兴项目(2022-SPY-00-019)
广东省现代农业产业技术体系(淡水鱼)创新团队建设项目(2023KJ150)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023SJHX4)共同资助。
关键词
华南鲤
血管内皮生长因子
侏儒
摄食
Cyprinus carpio rubrofuscus
Vascular endothelial growth factor
Dwarf
Feed intake
