摘要
目的比较4种消毒方法对环境表面的持续杀菌性能及成本,为临床环境消毒剂和消毒频次的选择提供实验依据。方法选用耐甲氧西林金黄色葡萄球菌(MRSA)为试验菌株,以不锈钢板模拟医院环境表面,用75%乙醇湿巾、季铵盐医用卫生湿巾、500 mg/L含氯消毒剂浸润抹布、1000 mg/L含氯消毒剂浸润抹布按照标准擦拭方法对不锈钢板进行擦拭消毒,分别在消毒前、消毒后即刻及30、60、90、120、150、240 min滴加试验菌悬液,作用5 min后采样并培养。比较4种消毒方法在不同时间的菌落总数和细菌杀灭率,统计消毒成本。结果与消毒前比较,4种消毒剂在消毒后各时间点的菌落数均降低(P<0.01);乙醇湿巾消毒后90min开始检出细菌菌落,与消毒后即刻比较差异有统计学意义(t=-3.6,P=0.02),消毒后150、180、240 min细菌菌落数逐渐增多,与消毒后120 min比较差异有统计学意义(t值分别为-13.2、-4.2、-8.2,P<0.01);双链季铵盐、500 mg/L含氯消毒剂均在消毒后120 min检出细菌菌落(t值分别为-3.5、-4.4,P<0.01),消毒后150、180、240 min细菌菌落数逐渐增多,与消毒后120 min比较差异有统计学意义(双链季铵盐组t值分别为-2.7、-7.9、-9.5,P<0.01;500 mg/L含氯消毒剂组t值分别为-3.2、-8.4、-9.8,P<0.01);1000 mg/L含氯消毒剂在消毒后150 min开始检出细菌菌落(t=-2.0,P=0.01),180、240 min细菌菌落数逐渐增多,与消毒后150 min比较差异有统计学意义(t分别为-2.0、-9.4,P<0.01)。消毒后即刻及30、60、240 min,4种消毒方法对环境表面细菌的杀菌率比较差异无统计学意义(P>0.05);乙醇湿巾消毒后90 min杀菌率降为95.1%,其他3种消毒方法杀菌率仍为100.0%(χ^(2)=11.4,P=0.01);消毒后120 min,1000 mg/L含氯消毒剂杀菌率仍为100.0%,其他3种消毒方法杀菌率均下降(χ^(2)=9.4,P=0.02);消毒后150 min,乙醇湿巾、500 mg/L含氯消毒剂杀菌率降至90.0%以下(χ^(2)=8.3,P=0.04);消毒后180min,乙醇湿巾�
Objective To compare the continuous disinfection ability and cost of 4disinfection methods on the surface,so as to provide an experimental basis for selecting disinfectants and determining disinfection frequencies in clinical environments.Methods Methicillin-resistant Staphylococcus aureus(MRSA)was selected as the tester strain,and stainless steel plates were used to simulate the surface of a hospital object.The 75%alcohol wipes,double-chain quaternary ammonium salt medical sanitary wipes,500mg/L chlorine disinfectant infiltration wipes,1000mg/L chlorine disinfectant infiltration wipes were used to wipe and disinfect the stainless steel plate according to the standard wiping method.The test bacterial suspension was applied before disinfection and at various time points after disinfection(0min,30min,60min,90 min,120min,150min,240min),with sampling and culturing performed after 5 minutes of exposure.The total number of bacterial colonies and bacterial killing rate on the environmental surface of the 4disinfection methods in different time periods was compared,and calculated the cost of four disinfectants.Results Compared with the pre-disinfection period,all 4disinfectants showed a decrease in the number of colonies at all time points after disinfection(P<0.01).Bacterial colonies began to be detected 90min after disinfection using ethanol wipes,and the difference was statistically significant when compared with the immediate post-disinfection period(t was-3.6,P=0.02),and the number of bacterial colonies increased gradually at 150,180,and 240min post-disinfection,and the difference was statistically significant when compared with 120min post-disinfection(t was-13.2,-4.2,-8.2,P<0.01);Bacterial colonies were detected at 120min after disinfection for both double-chain quaternary ammonium salt and 500mg/L chlorine-containing disinfectant(t was-3.5,-4.4,P<0.01),and the number of bacterial colonies gradually increased at 150,180,and 240min after disinfection,and the difference was statistically significant when compared wi
作者
邹朝双
高莹
罗炜
高园
郭明孝
王海涛
ZOU Chao-shuang;Gao Ying;LUO Wei;GAO Yuan;GUO Ming-xiao;WANG Hai-tao(Occupational and Environmental Health Monitoring Department,Dezhou City Center for Disease Control and Prevention,Shandong 253016,China;Bidding Office,Dezhou People's Hospital;Infection Management Department,Dezhou Maternal and Child Health Hospital)
出处
《预防医学论坛》
2024年第7期532-536,共5页
Preventive Medicine Tribune
基金
山东省德州市妇幼保健协会科技创新计划项目(DFY-202216)。
关键词
金黄色葡萄球菌
消毒
75%乙醇
双链季铵盐
消毒剂
环境表面
Staphylococcus aureus
Disinfection
75%ethanol
Double chain quaternary ammonium salt
Disinfectant
Environmental surface
