摘要
目的观察信号转导与转录激活因子3(STAT3)表达与早期非小细胞肺癌(NSCLC)淋巴结微转移之间的关系及其对患者预后的影响,探讨肿瘤微转移的新机制以及针对STAT3通路的靶向药物。方法收集2008年6月至2010年1月在山东第一医科大学附属省立医院50例行肺部分切除术的早期非小细胞肺癌患者和50例肺部良性肿瘤患者的肺组织和淋巴结样本。采用反转录聚合酶链反应检测MUC1mRNA在淋巴结样本中的表达及STAT3mRNA在NSCLC样本和正常肺组织样本中的表达,分析MUC1mRNA及STAT3mRNA表达量与NSCLC患者临床及病理特征的关系。采用蛋白印迹、免疫组织化学检测STAT3、pSTAT3蛋白在NSCLC样本及正常肺组织样本中的表达情况,分析STAT3、pSTAT3蛋白量表达与患者临床及病理特征的关系。采用Kaplan-Meier法绘制生存曲线,采用Logistic回归分析确定影响淋巴结微转移的独立危险因素,采用t检验或F检验进行分析。结果STAT3mRNA及蛋白表达与淋巴结微转移明显相关,MUC1阳性组中STAT3mRNA表达量高于阴性组(0.950±0.100比0.711±0.080,t=9.308,P<0.05);MUC1阳性组中STAT3蛋白表达量高于阴性组(1.086±0.078比0.860±0.085,t=9.427,P<0.05)。STAT3表达在NSCLC患者预后中的差异无统计学意义(χ^(2)=2.350,P>0.05)。Logistic回归分析显示,STAT3蛋白过表达[比值比(OR)=13.379,P<0.05]和肿瘤分化程度(OR=0.131,P<0.05)是淋巴结微转移的独立危险因素。结论STAT3过表达可能促进早期非小细胞肺癌的淋巴结微转移,并且可能作为淋巴结微转移的临床预测因子。
Objective To explore the relationship between signal transducer and activators of tran-scription 3(STAT3)expression and lymph node micrometastasis in early stage non-small cell lung cancer(NSCLC)and its effect on the prognosis of patients,so as to discover new mechanisms of tumor microme-tastasis and targeted drugs against STAT3 pathway.Methods Lung tissue and lymph node samples were collected from 50 patients with early-stage NSCLC and 50 patients with benign lung tumors who underwent partial lung resection at Shandong Provincial Hospital Affiliated to Shandong First Medical University from June 2008 to January 2010.Reverse transcription polymerase chain reaction was used to detect the expres-sion of MUC1 mRNA in lymph node samples and STAT3 mRNA in NSCLC samples and normal lung tissue samples.The relationship between the expression of MUC1 mRNA and STAT3 mRNA and the clinicopatho-logical characteristics of NSCLC patients was analyzed.Western blotting and immunohistochemistry was used to detect the expression of STAT3 and pSTAT3 in NSCLC samples and normal lung tissue samples,and the relationship between STAT3 and pSTAT3 protein expression and clinicopathological characteristics of patients was analyzed.The Kaplan-Meier method was applied to plot survival curves,and Logistic re-gression analysis was used to determine the independent risk factors for lymph node micrometastasis.Results The expression of STAT3 mRNA and protein was correlated with lymph node micrometastasis.The expression of STAT3 mRNA in MUC 1 positive group was higher than that in negative group(0.950±0.100 vs.0.711±0.080,t=9.308,P<0.05).Additionally,STA13 protein expression in MUC 1 posi-tive group was higher than that in negative group(1.086±0.078 vs.0.860±0.085,t=9.427,P<0.05).There was no significant difference in STAT3 expression in the prognosis of NSCLC patients(χ^(2)=2.350,P>0.05).Logistic regression analysis showed that STAT3 protein overexpression[odds ratio(OR)=13.379,P<0.05]and tumor differentiation(OR=0.131,P<0.05)were independen
作者
吴敬茹
季明浩
刘相燕
于洋
Wu Jingru;Ji Minghao;Liu Xiangyan;Yu Yang
出处
《中华实验外科杂志》
CAS
2024年第9期2087-2090,共4页
Chinese Journal of Experimental Surgery
基金
山东第一医科大学附属省立医院横向课题(6649c41b-855b-4739-9232-35934c8322c8)。
关键词
非小细胞肺癌
淋巴结转移
信号转导与转录激活因子3
磷酸化信号转导与转录激活因子3
黏蛋白1
Non-small cell lung cancer
Iymph node metastasis
Signal transducer and activators of transcription 3
phosphorylated signal transducer and activators of transcription 3
MUC 1
