摘要
目的研究苍耳草提取物对支气管哮喘大鼠肺功能及气道重构的影响及机制。方法选取无特定病原体级雄性SD大鼠60只,随机选取10只作为对照组,剩余50只以卵蛋白致敏法并雾化吸入诱导支气管哮喘模型。将预选合格的44只大鼠依据随机数字表法分为苍耳草低、中、高剂量组和模型组,各11只。苍耳低、中、高剂量分别以苍耳草乙醇提取物100、200、400 mg/kg灌胃,对照组和模型组以吐温、聚乙二醇、无菌0.9%氯化钠溶液混合物灌胃,连续干预4周。干预后检测各组大鼠肺功能;苏木精-伊红染色观察大鼠肺组织病理变化,肺泡灌洗液炎症细胞计数;反转录聚合酶链反应检测大鼠肺组织中核因子κB(NF-κB)/转化生长因子-β_(1)(TGF-β_(1))通路信使RNA(mRNA)表达。结果与对照组比较,模型组、苍耳草高及中剂量组0.3秒用力呼气量(FEV_(0.3))、用力肺活量(FVC)、FEV_(0.1)/FVC%和呼气峰流速均降低,且苍耳草高和中剂量组高于模型组(P<0.05)。苏木精-伊红染色结果显示,模型组支气管及管壁气道上皮细胞明显增生,黏膜水肿、增厚、管腔狭窄,大量炎症浸润;苍耳草干预组肺组织病理变化呈剂量依赖性减轻。与对照组比较,模型组和苍耳草中、高剂量组淋巴细胞、巨噬细胞、中性粒细胞和嗜酸粒细胞数量升高,且苍耳草中、高剂量组低于模型组(P<0.05)。与对照组比较,模型组和苍耳草中、高剂量组NF-κB p65、TGF-β_(1)mRNA相对表达量升高,且苍耳草中、高剂量组均低于模型组(P<0.05)。结论苍耳草提取物能改善支气管哮喘大鼠肺功能,抑制气道重构,其作用机制可能与抑制NF-κB/TGF-β_(1)信号通路的激活有关。
Objective To study the effect and mechanism of xanthium extract in lung function and airway remodeling in bronchial asthmatic rats.Methods Sixty male specific pathogen free SD rats were selected,and 10 were randomly selected as a control group,while the remaining 50 were sensitized with egg protein and nebulized inhalation to induce a bronchial asthma model.According to random number table method,44 pre-selected qualified rats were divided into a low-dose,a medium-dose and a high-dose xanthium group and a model group,with 11 rats in each group.The low,medium,high dose xanthium groups were intragastrically administered with 100,200,and 400 mg/kg of xanthium ethanol extract,while the control group and the model group were intragastrically administered with Tween,polyethylene glycol,sterile 0.9%sodium chloride solution mixture,all for 4 weeks of continuous intervention.After the intervention,the pulmonary function of each group was tested;hematoxylin eosin staining was used to observe the pathological changes of rat lung tissue;the inflammatory cells in alveolar lavage fluid were counted;reverse transcription polymerase chain reaction was used to detect the messenger RNA(mRNA)expression of nuclear factor-κB(NF-κB)/transforming growth factor-β_(1)(TGF-β_(1))pathway in rat lung tissue.Results Compared with the control group,the model group,xanthium medium and high dose group′s forced expiratory volume in 0.3 second(FEV_(0.3)),forced vital capacity(FVC),FEV_(0.1)/FVC%and peak expiratory flow all decreased,and the xanthium medium and high dose were higher than the model group(P<0.05).Hematoxylin eosin staining showed that the bronchus and airway epithelial cells of the model group were obviously proliferated,mucosal edema and thickening,narrowing of the lumen,and a large amount of inflammation infiltration occurred.The pathological changes of lung tissue in the xanthium interfering group were relieved in a dose-dependent manner.Compared with the control group,the number of lymphocytes,macrophages,neutrophils and e
作者
马静
姚弘毅
王模荣
MA Jing;YAO Hongyi;WANG Morong(Department of Critical Care Medicine,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China;Department of Rehabilitation Medicine,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China)
出处
《医学综述》
CAS
2022年第19期3914-3919,共6页
Medical Recapitulate
基金
湖北省卫生健康委员会中医药科研项目(ZY2019F039)。
关键词
支气管哮喘
苍耳草
气道重构
肺功能
Bronchial asthma
Xanthium
Airway remodeling
Pulmonary function
