摘要
为了解广西北海大风江水域环境对养殖香港牡蛎(Crassostrea hongkongensis)健康状况的影响,分别在2023年9—10月进行了3次调查研究,并对水体理化环境因子、浮游微藻种类、细菌丰度、香港牡蛎能量储备和氧化应激等指标进行了检测和分析。结果表明:9月河口水域温度为28.1℃,盐度为20.89,水体中总细菌和弧菌丰度分别为7.22×10^(8)、1.49×10^(7)cells/L,硅藻丰度较高(约为1.3×10^(7)cells/L),优势种为中肋骨条藻(Skeletonema costatum),养殖香港牡蛎鳃组织中丙二醛(MDA)含量为9.60 nmol/mg prot,闭壳肌中糖原和葡萄糖含量分别为6.18 mg/g和2.59μmol/g;10月河口水域温度下降,盐度上升,水体总细菌和弧菌丰度下降,分别为4.97×10^(7)、1.40×10^(4)cells/L,硅藻丰度下降至4.3×10^(5)cells/L,养殖香港牡蛎鳃组织MDA含量为1.02 nmol/mg prot,闭壳肌糖原和葡萄糖含量降低至2.49 mg/g和1.94μmol/g;10月河口上游水域温度和水体总细菌丰度与同期河口水域相近,但盐度和弧菌丰度均相对较低,硅藻丰度升高,养殖香港牡蛎的闭壳肌糖原和葡萄糖含量升高。研究表明,9月大风江河口水域养殖的香港牡蛎处于较高的氧化应激状态,可能与高温、高细菌和弧菌丰度等多重环境压力相关;10月河口水域养殖牡蛎的环境胁迫压力和氧化应激状态有所改善,但饵料藻类丰度的降低导致其糖原含量下降,应对环境变化的抵抗力减弱;10月大风江河口上游水域盐度低、饵料藻丰度高,养殖香港牡蛎处于相对健康状态。
To elucidate the impacts of water environment of Dafeng River in Beihai,Guangxi,on health status of Hong Kong oyster(Crassostrea hongkongensis)cultured therein,the physicochemical factors in water quality,planktonic microalgae,bacterial abundance,and energy reserve and oxidative stress of Hong Kong oysters were surveyed in the main Hong Kong oyster farming area of Beihai Sea of Guangzhou,the mouth of the Dafeng River and the upper waters(108°52′49″E,21°38′26″N;108°51′30″E,21°46′22″N)in September and October 2023.The results showed that,the estuarine water temperature was 28.1℃,with a salinity of 20.89,total bacterial abundance of 7.22×10^(8)cells/L and Vibrio abundance of 1.49×10^(7)cells/L in September.Diatom abundance was found to be approximately 1.3×10^(7)cells/L,with the dominant species of Skeletonema costatum.The malondialdehyde(MDA)content of 9.60 nmol/mg protein was observed in the gill of cultivated Hong Kong oysters,with glycogen content of 6.18 mg/g and glucose content of 2.59μmol/g in the adductor muscle.In October,however,the estuarine water temperature was decreased to 25.7℃,accompanied by an increase in salinity to 25.50,with bacterial abundance decreased to 4.97×10^(7)cells/L and Vibrio abundance to 1.40×10^(4)cells/L and diatom abundance decreased to 4.3×105 cells/L in the water.MDA content was 1.02 nmol/mg protein in the gill of cultivated Hong Kong oyster,and glycogen and glucose content in the adductor muscle was decreased to 2.49 mg/g and 1.94μmol/g,respectively.The water temperature and total bacterial abundance were similar to those in the estuarine waters in the upstream waters in October.However,salinity and Vibrio abundance were relatively low,with high diatom abundance.The glycogen and glucose contents in adductor muscle were increased in cultivated Hong Kong oysters.In summary,Hong Kong oysters cultured in the Dafeng River estuary faced multiple environmental stressors,including high temperature,elevated bacterial and Vibrio abundances in September,lead
作者
刘冉阳
高磊
张兴志
杨文文
赵俊彦
赵宝
于海杰
许吉祥
刘璐
彭金霞
王玲玲
宋林生
LIU Ranyang;GAO Lei;ZHANG Xingzhi;YANG Wenwen;ZHAO Junyan;ZHAO Bao;YU Haijie;XU Jixiang;LIU Lu;PENG Jinxia;WANG Lingling;SONG Linsheng(College of Life Sciences,Liaoning Normal University,Dalian 116029,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519000,China;Liaoning Key Laboratory of Marine Animal Immunology and Disease Control,Liaoning Key Laboratory of Marine Animal Immunology,Dalian Ocean University,Dalian 116023,China;Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Zhuang Autonomous Region Academy of Fishery Sciences,Nanning 530021,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2024年第4期551-558,共8页
Journal of Dalian Ocean University
基金
南方海洋科学与工程广东省实验室(珠海)资助项目(SML2023SP237)
国家自然科学基金(41961124009,32320110)
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-49)
农业农村部农业科研杰出人才及其创新团队项目。
关键词
香港牡蛎
大风江
水环境
能量储备
氧化应激
Crassostrea hongkongensis
Dafeng River
water environment
energy reserve
oxidative stress
