摘要
目的:基于独一味地上部分和根成分的差异性,探究独一味不同入药部位止血止痛的作用。方法:利用UHPLC-Q-Exactive-Orbitrap-MS快速表征和识别两种不同入药部位的化学成分。利用网络药理学挖掘不同入药部位独有成分及其与止血止痛功能相关的靶点,探究独一味地上部分和根独有成分止血止痛的药效物质基础并分析主要活性成分。结果:独一味地上部分鉴定出193个化合物,根中鉴定出191个化合物,其中地上部分和根部独有成分分别为13和11个。网络药理学结果表明发挥止血止痛作用的5个核心成分(甘草次酸、熊果酸、左旋千金藤啶碱、α-香附酮、冬凌草甲素)均来自地上部分,这些成分通过作用于177个交集基因并调控多条信号通路达到止血止痛的目的。结论:该研究表明独一味不同部位止血止痛的药效物质基础存在差异,独一味地上部分更适合作为止血止痛的入药部位,此结果可为独一味不同部位的开发利用及资源保护提供依据。
Objective:Based on the different compositions between aboveground part and root of Lamiophlomis rotata,the hemostatic and analgesic effects of these two different parts in L.rotata were explored.Methods:UHPLC-Q-Exactive-Orbitrap-MS was used to quickly characterize and identify the chemical compositions of two different parts.The network pharmacology was used to study the unique components of different medicinal parts and the related targets associated with hemostasis and analgesia,explore the material basis acting on hemostasis and analgesia of the unique components of aboveground part and root,and to analyze the main active components in the L.rotata.Results:193 compounds were identified in the aboveground part and 191 compounds were identified in the roots of L.rotata,of which 13 and 11 were unique components of the aboveground part and roots,respectively.The results of network pharmacology indicated that the five core components(18β-Glycyrrhetintic acid,Ursolic acid,l-Stepholidine,α-Cyperone,Rubescensin A),which exerted the hemostatic and analgesic effects,were all from the aboveground part of the plant.These components achieved hemostasis and analgesia through their actions on the 177 intersecting genes and the modulation of multiple signaling pathways.Conclusion:The study showed that the pharmacodynamic material basis for treating hemostasis and analgesia of different parts of L.rotata was different,and the aboveground part was more suitable for treating hemostasis and analgesia as medicine of L.rotata,which providing a basis for the development and utilization of different parts and resource protection about L.rotata.
作者
徐丽
陈海娟
张国燕
尚军
Xu Li;Chen Haijuan;Zhang Guoyan;Shang Jun(Key Lab.of Medicinal Animal and Plant Resources in Qinghai Tibet Plateau,School of Life Sciences,Qinghai Normal University,Xining 810008,China;Academy of Plateau Science and Sustainability,Xining 810008,China)
出处
《中国野生植物资源》
CSCD
2024年第8期10-20,28,共12页
Chinese Wild Plant Resources
基金
青海省科技厅国际合作项目(2021-HZ-805)
青海省“昆仑英才·高端创新创业人才”项目(2020)。
