摘要
WRKY转录因子在植物逆境防御中起着重要作用,它们参与生物胁迫与非生物胁迫的调控。本研究以青花菜为材料,在克隆转录因子基因BoiWRKY7的基础上,开展序列分析和系统发育分析,并明确其在野油菜黄单胞菌(Xanthomonas campestris pv.campestris)和霜霉菌(Hyaloperonospora parasitica)侵染下的表达模式。结果表明,BoiWRKY7的DNA全长为1416 bp,具2个内含子,长度分别为94和308 bp;基因的编码区全长为1014 bp,编码337个氨基酸;系统发育分析结果表明,BoiWRKY7与野甘蓝WRKY7聚为一组,支持率达100%;基因表达分析结果表明,BoiWRKY7的表达受野油菜黄单胞菌的诱导,表达水平在24和48 h时的表达量最大;BoiWRKY7的表达还受霜霉菌的诱导,在48 h时的表达量最大。青花菜BoiWRKY7的克隆与表达研究,为将来开展该基因的功能鉴定提供了基础。
WRKY transcription factors play an important role in plant stress defense,and they participate in the regulation of biotic and abiotic responses.In the study,the aim is to isolate a WRKY transcription factor,namely BoiWRKY7,from Brassica oleracea var.italica,and to obtain its expression patterns challenged by Xanthomonas campestris pv.campestris(Xcc)and Hyaloperonospora parasitica.The results showed that the full genomic DNA of BoiWRKY7 was 1416 bp,containing two introns of 94 and 308 bp in length;the complete coding sequence was1014 bp,encoding 377 amino acids;phylogenetic analysis indicated that BoiWRKY7 was grouped with WRKY7from B.oleracea,with a support rate of 100%;expression analysis revealed that the expression of BoiWRKY7 was induced by Xcc,and the highest expression levels were observed at 24 and 48 h after pathogen inoculation.Additionally,BoiWRKY7 was induced by H.parasitica,reaching the highest level at 48 h.Isolation and expression analysis of BoiWRKY7 laid a basis for future investigation on functional identification of broccoli disease resistance responses.
作者
朱晏
蒋明
张慧娟
黄雯馨
吴倩
周梦亚
张胜
Zhu Yan;Jiang Ming;Zhang Huijuan;Huang Wenxin;Wu Qian;Zhou Mengya;Zhang Sheng(College of Life Science,Taizhou University,Taizhou,318000;Taizhou Agricultural Technology Extension and Service Center,Taizhou,318000)
出处
《分子植物育种》
CAS
北大核心
2024年第19期6281-6288,共8页
Molecular Plant Breeding
基金
台州市科技计划项目(1901ny08)
浙江省自然科学基金项目(LY19C150004)共同资助。
