摘要
植物需要利用太阳光能进行光合作用,因而不可避免地受到紫外线-B(UV-B)辐射的影响.为了鉴定水稻WRKY转录因子OsWRKY89基因启动子中的UV-B反应相关元件,分析了转启动子不同缺失片段与gus融合基因的水稻幼苗,发现在该启动子中存在UV-B反应元件,位于基因翻译起始位点上游-1213～-1188之间的25bp区域,碱基序列为AAGATCTACCATTGCTCTATAGCTT.结合OsWRKY89和UV-B诱导上调表达基因启动子序列分析发现,该元件区在水稻UV-B反应基因启动子上具有高度的保守性,而且与已知保守的光反应元件位置邻近,表明该区域在水稻UV-B反应的转录调控中可能具有重要功能.
OsWRKY89, a rice WRKY transcription factor gene, is induced by UV-B radiation. To identify the cis-elements in the OsWRKY89 promoter related to UV-B response, the promoter and its deleted fragments were each fused with gus reporter gene and transformed into rice calli. The transgenic plants regenerated were used to analyze their responses to UV-B irradiation. Finally, an UV-B response cis-regulatory element was narrowed to the region of 25 bp between -1 188 and -1 213 upstream the translation start site of OsWRKY89 gene, including nucleotide sequence of AAGATCTACCATTGCTCTATAGCTT. Through analysis of the promoter of OsWRKY89 and 28 genes up-regulated by UV-B treatment, two relatively conserved UV-B response elements in the 1 500 bp protmoter region studied were located. Coincidental, the 25 bp of OsWRKY89 promoter contains one of the conserved elements. Furthermore, several conserved light-response elements including ACEOsWRKY89, MREOsWRKY89 and UVBoxAtANAC13 were also found to adjoine to the 25 bp UV-B response region in the OsWRKY89 promoter, suggesting they together play roles in regulating the light responses of the gene.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2010年第6期671-677,共7页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(30771393)~~
