摘要
【目的】前期研究显示苏云金芽胞杆菌(Bacillus thuringiensis,Bt)4BM1菌株可诱导油菜产生对核盘菌(Sclerotinia sclerotiorum)的系统抗病性。探究其诱导系统抗性机制并分析其生防潜力,为油菜菌核病的生物防治提供菌株资源。【方法】采用实时荧光定量PCR和转录组测序技术分析根部施用4BM1菌株后,油菜叶片中防御相关基因的转录水平;结合4BM1菌株全基因组序列和antiSMASH 2.0软件预测其抗病相关次级代谢产物生物合成的基因簇;采用发酵液灌根的方式将4BM1菌株接种至油菜盆栽幼苗,分析其定殖能力和促生效果。【结果】4BM1菌株可激发油菜叶片产生过敏反应;油菜根部施用4BM1菌株,叶片中参与水杨酸、茉莉酸、乙烯信号和油菜素内酯生物合成途径等基因转录水平显著上调;4BM1菌株的染色体上预测到胞外多糖和其他13个抗病相关次级代谢产物合成的基因簇;4BM1菌株在油菜根际定殖的同时可促进油菜苗期植株的生长。【结论】4BM1菌株可作为防控油菜菌核病、促进油菜幼苗生长有潜力的生物防治资源。
【Objective】In the previous study,Bacillus thuringiensis 4BM1 strains effectively suppressed Sclerotinia sclerotiorum growth by inducing systemic resistance in Brassica campestris.The induced systemic resistance mechanism and biocontrol potential were studied,which may provide strain resources for the biological control of sclerotiniose.【Method】Quantitative real-time PCR and transcriptome sequencing were used to analyze the transcription levels of defense-related genes in B.campestris leaves after roots were exposed to the Bt 4BM1 strain.The disease-resistance related secondary metabolites biosynthesis gene clusters were predicted by using the complete genome sequence of Bt 4BM1 strain and the antiSMASH 2.0 software.The 4BM1 strain was inoculated into the potted B.campestris seedlings by fermentation liquid irrigation method,and its colonization ability and growth promotion effect were analyzed.【Result】4BM1 strain triggered hypersensitive response in B.campestris leaves.Genes involved in salicylic acid,jasmonic acid,ethylene signaling and brassinosteroid synthesis pathways of B.campestris leaves were upregulated after the roots were exposed to the Bt 4BM1 strain.The candidate gene clusters involved in exopolysaccharide and 13 other disease-resistance related secondary metabolites biosynthesis were predicted in 4BM1 strain chromosome.The 4BM1 strain colonized in the rhizosphere of B.campestris and promoted the growth of B.campestris.【Conclusion】4BM1 strain can be used as a potential biological control resource to control sclerotiniose and promote the growth of B.campestris seedlings.
作者
王美玲
耿丽丽
房瑜
束长龙
张杰
WANG Mei-ling;GENG Li-li;FANG Yu;SHU Chang-long;ZHANG Jie(College of Plant Protection,Shanxi Agricultural University,Taigu 030801;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第9期260-269,共10页
Biotechnology Bulletin
基金
山西省基础研究计划(自由探索类)(202203021212460)
山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目(SXBYKY2022062)。
