摘要
为探讨纳豆饲料对抗生素性腹泻小鼠肠道菌群结构和肠道稳态失调的保护作用,向C57BL/6小鼠连续饲喂2.5%添加量的纳豆饲料19周后,通过连续灌胃复合抗生素7 d[110 mg/mL,0.4 mL/(d·只)]建立抗生素性腹泻模型(Antibiotic-associated diarrhea,AAD),随后停用抗生素,恢复自由饮食7d。测定造模后和恢复后小鼠生理指标,肠道损伤情况和肠道菌群结构功能的变化。结果显示,食用纳豆能够缓解AAD小鼠的体重降低,结肠缩短和结肠病理损伤,还可以通过改善血清炎症因子、血清内毒素和结肠免疫球蛋白水平的异常,升高结肠SOD含量,降低结肠MDA含量,减轻AAD小鼠炎症反应和氧化损伤。此外,食用纳豆增加了AAD小鼠结肠短链脂肪酸水平,增加了Bacillus、Enterococcus、Allobaculum等有益菌丰度。这些菌群结构的变化与纳豆改善的AAD小鼠病理指标显著相关,且显著加速了AAD小鼠的指标恢复。本研究结果表明,食用纳豆能够对AAD小鼠的氧化应激、炎症水平、肠道菌群结构和肠道稳态产生有益作用,从而保护小鼠抵御抗生素造成的肠道损伤。
To investigate the protective effect of natto feed on intestinal flora and intestinal homeostasis in mice with antibiotic-induced diarrhea.C57BL/6 mice were fed with 2.5%natto for 19 weeks,and the model of antibiotic-induced diarrhea(AAD)was established by intragastric administration of compound antibiotics for 7 days[110 mg/mL,0.4 mL/(d per)],antibiotics were then stopped and the free diet resumed for 7 days.The physiological indexes,intestinal injury and the changes of intestinal flora structure and function were determined after modeling and recovery.The results showed that long-term consumption of natto could alleviate the weight loss,colon shortening and pathological damage in AAD mice.The natto diet also increases colon SOD levels by improving abnormal levels of serum inflammatory factors,endotoxin and colonic immunoglobulins,decreasing the content of MDA in AAD mice colon.More importantly,long-term consumption of natto increased colon short-chain fatty acid levels and increased the abundance of beneficial bacteria such as Bacillus,Enterococcus and Allobaculum in AADmice.The changes of these flora structures are significantly related to the pathological indexes of AADmice improved by natto,and natto significantly accelerates the recovery of AADmice.Long-termconsumption of natto can have beneficial effects on oxidative stress,inflammation level,intestinal flora structure and intestinal homeostasis in AAD mice,thus protecting mice fromintestinal injury caused by antibiotics.
作者
黄伟
张雪聪
何宇光
苗欣宇
孙慕白
牛红红
李达
苏颖
甘雪彤
华梅
王景会
HUANG Wei;ZHANG Xuecong;HE Yuguang;MIAO Xinyu;SUN Mubai;NIU Honghong;LI Da;SU Ying;GAN Xuetong;HUA Mei;WANG Jinghui(College of Life Sciences,Jilin Normal University,Siping 136000,China;Institute of Agro-food Technology,Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Changchun 130033,China;College of Food Science and Engineering,Jilin Agricultural University,Changchun 130033,China)
出处
《特产研究》
2024年第5期8-17,共10页
Special Wild Economic Animal and Plant Research
基金
吉林省农业科学院基本科研经费项目(KYJF2023JJ103)
吉林省农业科技创新工程项目引进博士及高层次项目(CXGC2022RCG001)
吉林省农业科技创新工程项目研究生基金(CXGC2022RCY023、CXGC2022RCY026)。
关键词
纳豆
氧化应激
炎症因子
抗生素性腹泻
肠道稳态
natto
oxidative stress
inflammatory factors
antibiotic diarrhea
intestinal homeostasis
