摘要
Xylooligosaccharide(XOS)has been considered to be an effective prebiotic,but its exact mechanisms remain unknown.This research was conducted to evaluate the effects of XOS on pig intestinal bacterial community and mucosal barrier using a lipopolysaccharide(LPS)-caused gut damage model.Twenty-four weaned pigs were assigned to 4 treatments in a 2×2 factorial design involving diet(with or without XOS)and immunological challenge(saline or LPS).After 21 d of feeding 0%or 0.02%commercial XOS product,piglets were treated with saline or LPS.After that,blood,small intestinal mucosa and cecal digesta were obtained.Dietary XOS enhanced intestinal mucosal integrity demonstrated by higher villus height,villus height-to-crypt depth ratio,disaccharidase activities and claudin-1 protein expression and lower crypt depth.XOS also caused down-regulation of the gene expression of toll-like receptor 4 and nucleotide-binding oligomerization domain protein signaling,accompanied with decreased pro-inflammatory cytokines and cyclooxygenase 2 contents or mRNA expression and increased heat shock protein 70 mRNA and protein expression.Additionally,increased Bacteroidetes and decreased Firmicutes relative abundance were observed in the piglets fed with XOS.At the genus level,XOS enriched the relative abundance of beneficial bacteria,e.g.,Faecalibacterium,Lactobacillus,and Prevotella.Moreover,XOS enhanced short chain fatty acids contents and inhibited histone deacetylases.The correlation analysis of the combined datasets implied some potential connections between the intestinal microbiota and pro-inflammatory cytokines or cecal metabolites.These results suggest that XOS inhibits inflam-matory response and beneficially modifies microbes and metabolites of the hindgut to protect the in-testine from inflammation-related injury.
基金
funded by the Projects of Innovative Research Groups of the Natural Science Foundation of Hubei Province(grant number 2019CFA015)
National Natural Science Foundation of China(grant number 31802070 and 31772615)
Wuhan Science and Technology Bureau(grant number 2018020401011304)
