摘要
目的运用生物信息学筛选星形细胞瘤的相关核心基因。方法从GEO数据库下载星形细胞瘤表达数据集GSE70231和GSE138999,采用RStudio分析后得到差异表达基因(DEGs)。对DEGs进行GO和KEGG富集分析,应用STRING网站构建蛋白互作网络(PPI)后导入Cytoscape筛选核心基因。利用Sangerbox平台分析核心基因在肿瘤中的表达和对生存预后的影响。最后,利用RStudio对数据进行免疫浸润分析。结果RStudio分析共1261个DEGs;富集分析发现差异基因主要位于质膜和细胞质,参与化学突触传递和蛋白质结合等过程,与突触囊泡循环通路和胰岛素分泌通路相关。Cytoscape筛选出3个核心基因,分别是SNAP25、SYN1、SYT1。Sangerbox分析显示肿瘤与正常组织的核心基因表达存在明显的差别,并且高表达的核心基因整体生存相对较好。免疫浸润分析发现星形细胞瘤组织中,巨噬细胞(M1型和M2型)和中性粒细胞等浸润程度较高,而不同免疫细胞间也存在一定相关性。结论SNAP25、SYN1、SYT1可能在星形细胞瘤发展过程中发挥作用,有望成为星形细胞瘤的潜在治疗靶点。
Objective To identify the core genes related to astrocytoma using bioinformatics.Methods Expression datasets GSE70231 and GSE138999 of astrocytoma were downloaded from the Gene Expression Omnibus(GEO)database and Differentially Expressed Genes(DEGs)were identified using RStudio.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed on the DEGs.The Protein-protein Interaction(PPI)network was constructed using STRING and imported into Cytoscape to screen for core genes.The expression of core genes in tumors and their impact on survival prognosis were analyzed using the Sangerbox platform.Finally,immune infiltration analysis was conducted using RStudio.Results A total of 1261 DEGs were identified through RStudio analysis.Enrichment analysis revealed that these DEGs were mainly localized to the plasma membrane and cytoplasm,involved in processes such as chemical synaptic transmission and protein binding,and associated with synaptic vesicle cycle and insulin secretion pathways.Three core genes,SNAP25,SYN1,SYT1,were identified using Cytoscape.Sangerbox analysis showed significant differences in core gene expression between tumor and normal tissues,with high expression of core genes being associated with better overall survival.Immune infiltration analysis in astrocytoma tissues revealed high levels of infiltration of macrophages(M1 and M2)and neutrophils,with some correlation observed among various immune cells.Conclusion SNAP25,SYN1 and SYT1 may play important roles in the progression of astrocytoma and hold potential as therapeutic targets.
作者
雷丽巧
刘建民
邓知敏
Lei Liqiao;Liu Jianmin;Deng Zhimin(The First Clinical Medical College,Guangzhou University of Chinese Medicine,Guangzhou 510000,Guangdong,China;Baiyun Hospital of the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000,Guangdong,China)
出处
《生物医学转化》
2024年第3期47-54,共8页
Biomedical Transformation
基金
广州市科技计划项目(201607010365)。
