摘要
目的:基于超高效液相色谱-四级杆飞行时间质谱法(UPLC Q-TOF MS)对比不同种百合(卷丹及百合)的新鲜品和干燥品(冷冻干燥及烘干)成分的差异。方法:采用UPLC Q-TOF MS技术,对不同种百合的新鲜品和干燥品进行分析,进一步通过主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)结合单因素分析筛选百合新鲜样品及烘干样品的差异化合物。结果:UPLC Q-TOF MS结果显示,百合新鲜样品和干燥品主要成分相似。PCA结果显示,百合新鲜品与冻干品聚为一类,而两者与烘干品有明显差异,说明百合冻干样品和新鲜样品整体化学成分相近,而烘干样品经过烫片和烘干处理后化学成分发生了一定变化,故与前两者相异。OPLS-DA结果显示,卷丹新鲜品与烘干品存在387个差异成分,百合新鲜品与烘干品存在335个差异成分,经数据库比对,初步鉴定出8个成分。结论:相较于烘干百合,鲜百合与冷冻干燥百合成分更为相近,为百合药材临床应用与现代中药制剂开发提供依据。
Objective:To compare the differences in components between fresh and dried(freeze-dried and ovendried)samples of different species of Lilii Bulbus(Lilium lancifolium Thunb.and L.brownii F.E.Brown var.viridulum Baker)based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC Q-TOF MS).Methods:UPLC Q-TOF MS technology was employed to analyze fresh and dried samples of different Lilii Bulbus species.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)combined with single-factor analysis were employed to screen for differential compounds in fresh and oven-dried Lilii Bulbus samples.Results:The UPLC Q-TOF MS showed that the main components of fresh and dried Lilii Bulbus samples were similar.PCA results showed that fresh and freeze-dried Lilii Bulbus samples clustered into one group,while both differed significantly from oven-dried samples.This suggests that the overall chemical compounds of freeze-dried Lilii Bulbus samples are similar to fresh samples.In contrast,the chemical composition of oven-dried samples changed after blanching and drying,making them different from the former two.OPLS-DA results showed 387 differential components between fresh and oven-dried Lilium lancifolium Thunb.samples and 335 differential components between fresh and oven-dried Lilium Bulbus samples.8 components were preliminarily identified through database comparison.Conclusion:Compared to oven-dried Lilium Bulbus,the components of fresh and freeze-dried Lilii Bulbus are more similar,providing a basis for the clinical application and modern Chinese medicine formulation development of Lilii Bulbus.
作者
王碧莹
张欣亚
陈随清
付钰
WANG Bi-ying;ZHANG Xin-ya;CHEN Sui-qing;FU Yu(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province&Education Ministry of P.R.China,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中国现代中药》
CAS
2024年第9期1511-1519,共9页
Modern Chinese Medicine
基金
河南省中医药科学研究专项课题重大专项(2022ZYZD18)
河南省高等学校重点科研项目(24A360004)。
