摘要
为了解新疆和田地区鹅星状病毒(goose astrovirus,GAstV)的感染情况和分子遗传学特征,无菌采集和田县、于田县和皮山县3个鹅场死亡雏鹅的内脏及肛拭子,通过RT-PCR对GAstV进行检测;应用LMH细胞进行病毒分离鉴定,并对分离株进行全基因组测序与遗传特征分析。结果显示:GAstV在上述地区的总阳性率为11.25%(65/578);通过病毒分离鉴定出2株GAstV,命名为GAstV/XJHT-1和GAstV/XJHT-2。序列测定显示2株病毒的基因组长为7190、7125bp;全基因组同源性分析显示2个分离株均与GAstV-1和GAstV-2的同源性均高于95%,与其他来源(鸡、鸭、火鸡)的同源性为54.1%~61.5%。遗传进化分析显示,分离的GAstV与河南和安徽的GAstV分离株遗传距离较近,表明分离株GAstV可能源于上述两地引进的雏鹅苗或鹅蛋。本试验为进一步研制疫苗或防控产品提供了依据。
In order to understand the infection and molecular genetic characteristics of goose astrovirus(GAstV)in Hotan,Xinjiang,visceral organs and swabs of dead goslings were collected aseptically from three goose farms in Hotan,Yutian and Pashan counties,and GAstV was detected by RT-PCR.The positive samples were screened and identified in LMH cells,and the whole genome was sequenced,and the genetic characteristics of the isolates were analyzed.The results showed that the total positive rate of GAstV was 11.25%(65/578).Two strains of GAstV named as GAstV/XJHT-1and GAstV/XJHT-2were isolated and the lengths of their genome sequences were determined as 7190bp and 7125bp,respectively.Whole genome homology analysis showed that the homology of the two isolates with GAstV-1and GAstV-2was higher than 95%,and the homology with other sources(chicken,duck,and turkey)ranged from 54.1%to 61.5%.Genetic evolution analysis showed that the genetic distance between GAstV isolates from Henan and Anhui was relatively close,suggesting that the isolated GAstV may be related to the introduction of goslings or goose eggs from these two places.The findings provide a basis for further development of vaccines or control products.
作者
肖海侠
张凌
王燕
马万鹏
阿斯也木·亚森
高锦
苏战强
XIAO Haixia;ZHANG Ling;WANG Yan;MA Wanpeng;ASIYEMU·Yasen;GAO Jin;SU Zhanqiang(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Livestock and Veterinary Station,Hongqi Town,Shayar County,Aksu,Xinjiang 842205,China;Kunlun Lyuyuan Goose Breeding Technology Limited Company,Hotan,Xinjiang845150,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第7期1401-1407,共7页
Chinese Journal of Veterinary Science
基金
自治区重大科技专项资助项目(2022273358)
自治区畜禽种业提升资助项目(2023xjjq-05)。
关键词
鹅星状病毒
分离鉴定
全基因组测序
同源性
遗传进化
goose astrovirus
isolation and identification
whole genome sequencing
homology
genetic evolution
