摘要
[目的]建立高效液相色谱-紫外检测器(HPLC-UV)法测定银杏内酯A、B、C的浓度,并对其进行方法学验证。[方法]考察了包括检测波长、8种不同的流动相体系及其比例、进样量、流速和柱温。方法验证涵盖了专属性、精密度、准确度、线性范围、重复性、系统适用性、加样回收率和稳定性。[结果]色谱柱为Agilent TC-5 C_(18),检测波长为220 nm,流动相为水-甲醇(70∶30),进样量为20μL,柱温30℃,流速为1.0 mL/min且线性关系良好,色谱峰之间无互相干扰。[结论]该方法准确、有效,经济且易得,操作简单,为其他银杏内酯药物含量测定提供了可靠的参考。
[Objective]To establish and methodologically validate an HPLC-UV method for the determination of ginkgolides A,B,and C.The method was validated by the HPLC-UV method.[Methods]The investigation included detection wavelength,eight different mobile phase systems and their ratios,injection volume,flow rate and column temperature.The method validation examined the specificity,precision,accuracy,linear range,repeatability,system suitability,spiked recovery and stability.[Results]The separation was carried out on an Agilent TC-5 C_(18) column at a detection wavelength of 220 nm with a mobile phase of water-methanol(70∶30)at an injection volume of 20μL,a column temperature of 30℃,and a flow rate of 1.0 mL/min.The linearity of the method was good,and the peaks of the chromatograms did not interfere with each other.[Conclusion]The method is accurate,effective,economical,easy to obtain and simple to operate,and can provide a reference for the determination of other ginkgolides.
作者
胡哲铭
皮紫月
JOHN OSILAMA THOMAS
郭盼
HU Zheming;PI Ziyue;JOHN OSILAMA THOMAS;GUO Pan(Modern Chinese Medicine Discovery and Preparation Technology Engineering Center of the Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《天津中医药》
CAS
2024年第9期1182-1186,共5页
Tianjin Journal of Traditional Chinese Medicine
