摘要
目的:探索黄芩苷抗子宫平滑肌瘤细胞增殖、迁移和侵袭的影响及其可能的机制。方法:体外培养原代子宫平滑肌瘤细胞,并通过α-Actin免疫染色鉴定。用5、10和20μmol/L的黄芩苷干预后,通过CCK8检测细胞的存活率;通过划痕和Transwell实验检测细胞的迁移和侵袭;通过Hoechst染色检测细胞的凋亡;通过蛋白质印迹法(Western Blot)检测细胞中凋亡相关蛋白Bax、Bcl-2及PI3K/AKT信号通路蛋白的表达。结果:α-Actin免疫染色鉴定了原代子宫平滑肌瘤细胞的成功提取。CCK8实验结果显示,10和20μmol/L的黄芩苷能够剂量依赖性地抑制子宫平滑肌瘤细胞的生长。划痕和Transwell实验结果显示,10和20μmol/L黄芩苷能够显著抑制子宫平滑肌瘤细胞的迁移和侵袭。Hoechst染色显示10和20μmol/L黄芩苷明显促进了子宫平滑肌瘤细胞的凋亡;20μmol/L黄芩苷显著了促进了凋亡蛋白Bax的表达,显著抑制了抗凋亡蛋白Bcl-2的表达。此外,20μmol/L黄芩苷显著抑制了子宫平滑肌瘤细胞中NF-κB、p-AKT和p-PI3K的表达,显著抑制了PI3K/AKT信号通路的磷酸化。结论:黄芩苷可以显著抑制子宫平滑肌瘤细胞的增殖、迁移和侵袭,其机制可能与抑制PI3K/AKT信号通路和IκB磷酸化有关。
Objective:To explore the possible mechanism of baicalin against proliferation,migration and invasion of uterine leiomyoma cells.Methods:Primary uterine leiomyoma cells were cultured in vitro and identified byα-Actin immunostaining.After the intervention of baicalin with 5,10 and 20μmol/L,the survival rate of cells was detected by CCK8.The migration and invasion of cells were detected by scratch and Transwell assay.Hoechst staining was used to detect cell apoptosis.The expression of apoptosis-related proteins Bax,Bcl-2 and PI3K/AKT signaling pathway was detected by Western Blot.Results:α-Actin immunostaining identified the successful extraction of primary uterine leiomyoma cells.The results of CCK8 experiment showed that baicalin at 10 and 20μmol/L could inhibit the growth of uterine leiomyoma cells in a dose-dependent manner.The results of scratch and Transwell experiments showed that 10 and 20μmol/L baicalin could significantly inhibit the migration and invasion of uterine leiomyoma cells.Hoechst staining showed that 10 and 20μmol/L baicalin significantly promoted the apoptosis of uterine leiomyoma cells.Baicalin at 20μmol/L significantly promoted the expression of apoptotic protein Bax,and significantly inhibited the expression of anti-apoptotic protein Bcl-2.In addition,20μmol/L baicalin significantly inhibited the expression of NF-κB,p-AKT and p-PI3K in uterine leiomyoma cells,and significantly inhibited the phosphorylation of PI3K/AKT signaling pathway.Conclusion:Baicalin significantly inhibited the proliferation,migration and invasion of uterine leiomyoma cells,and its inhibitory effect was related to the regulation of PI3K/AKT signaling pathway and phosphorylation of IκB.
作者
赖振岸
林泽辉
王明霞
徐雪怡
LAI Zhen'an;LIN Zehui;WANG Mingxia;XU Xueyi(Medical Rehabilitation Department,Hainan Anning Hospital,Hainan Haikou 570000,China;Emergency Department,Hainan Traditional Chinese Medicine Hospital,Hainan Haikou 570100,China;Department of Neurology,Hainan Anning Hospital,Hainan Haikou 570000,China)
出处
《现代肿瘤医学》
CAS
2024年第18期3441-3447,共7页
Journal of Modern Oncology
