摘要
为了提升脱除剂的吸附水平和可重复利用能力,并用于脱除黄曲霉毒素B_(1)(AFB_(1)),利用十六烷基三甲基溴化铵(CTAB)改性处理蒙脱石(MMT-CTAB),结合固定化漆酶(Laccase)制得改性蒙脱石固定化漆酶(MMT-CTAB-Lac),利用方程拟合考察脱除剂的吸附动力学,优化MMT-CTAB-Lac脱除AFB1最适条件,并利用LC-MS和T.E.S.T软件探究AFB1降解途径及其降解产物毒性。改性处理后的脱除剂具有更大层间距和孔容、更小颗粒粒径,吸附AFB_(1)的比表面积增大;吸附于脱除剂中的AFB_(1)被固定化漆酶降解,有效阻止了AFB_(1)在脱除剂上的聚集,显著增大了MMT-CTAB-Lac吸附容量;化学吸附是AFB1吸附过程中限速步骤,MMT-CTAB-Lac对AFB_(1)的吸附量为44.5 mg·g^(-1),高于MMT-CTAB的吸附量;MMT-CTAB-Lac对AFB_(1)脱除率为67%,且在弱酸及中性条件下均具有良好催化降解效果。进一步鉴定了MMT-CTAB-Lac降解AFB1的产物,并提出了其降解AFB1的两种可能途径:第1种可能降解途径(内酯环结构被破坏),降解产物为C17H14O7和C16H12O5;第2种可能降解途径(呋喃环结构被破坏),降解产物为C17H14O7、C16H12O7和C14H10O4。并通过T.E.S.T软件的毒性预测,得出降解产物毒性有所降低。本研究开发的MMT-CTAB-Lac为粮油食品中AFB_(1)的安全、高效脱除奠定了重要基础。
In this study,the removal agent of modified montmorillonite immobilized laccase(MMT-CTAB-Lac)was prepared for the removal of Aflatoxin B_(1)(AFB_(1)).After the treatment of cetyltrimethyl ammoni-um bromide(CTAB),the MMT-CTAB-Lac was prepared by the modified montmorillonite(MMT-CTAB)with the immobilized laccase.The adsorption kinetics of the removal agent was investigated by the equation fitting.The conditions for removing AFB_(1) from the MMT-CTAB-Lac were optimized.The AFB_(1) degradation pathway and the toxicity of its degradation products were investigated by the LC-MS and T.E.S.T software.After the modification,the remover had larger layer spacing and pore volume,smaller parti-cle size,and the increased specific surface area for the AFB_(1) adsorption.The AFB_(1) adsorbed on the remov-er was degraded by the immobilized laccase,which effectively prevented the accumulation of AFB_(1) on the remover,and significantly increased the adsorption capacity of MMT-CTAB-Lac.The chemisorption was a rate-limiting step in the adsorption process of AFB_(1).The adsorption capacity of MMT-CTAB-Lac for AFB_(1) was 44.5 mg·g^(-1),which was higher than that of MMT-CTAB.The removal rate of AFB_(1) by the MMT-CTAB-Lac was 67%,with the high catalytic degradation rate under the weak acid and neutral con-dition.The degradation of AFB_(1) by MMT-CTAB-Lac was further identified.The two possible degradation pathways were proposed:the first possible degradation pathway(destruction of lactone ring structure),with the degradation products of C17H14O7 and C16H12O5;the second possible degradation pathway(de-struction of furan ring structure),with the degradation products of C17H14O7,C16H12O7,and C14H10O4.Through the toxicity prediction of T.E.S.T software,the toxicities of degradation products were reduced.The MMT-CTAB-Lac developed in this study lays an important foundation for the safe and efficient removal of AFB_(1) in the field of grain,oil and food.
作者
邬有胜
王乐
张孟月
梁赢
王金水
WU Yousheng;WANG Le;ZHANG Mengyue;LIANG Ying;WANG Jinshui(School of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2024年第4期64-74,共11页
Journal of Henan University of Technology：Natural Science Edition
基金
河南省自然科学基金项目(232103810065)
河南省重点研发专项(221111320500)
河南省高校科技创新人才支持计划(20HASTIT034)
河南省“双一流”创建项目(食品科学与工程学科方向)。
