摘要
目的:评估表面修饰光动力抗菌聚合物钛片(Ti-PGMB)植入体的抗感染性能。方法:用活性氧(ROS)荧光探针验证在光照条件下Ti-PGMB植入体产生ROS,分别建立耐甲氧西林金黄色葡萄球菌(MRSA)生物膜模型及植入体部位感染动物模型评估Ti-PGMB抗细菌感染的效果。将小鼠随机分为钛片对照(Ti)组、Ti-PGMB无光照(Ti-PGMB-L)组和Ti-PGMB光照(Ti-PGMB+L)组,每组各10只。Ti组植入钛片,Ti-PGMB+L组和Ti-PGMB-L组植入Ti-PGMB。在植入位置接种等量MRSA细菌悬液(5×10^(7)CFU/mL),24 h后对Ti-PGMB+L组连续光照干预7 d,Ti组和Ti-PGMB-L组不进行其他干预措施。观察3组小鼠植入体部位缝合处切口恢复情况,统计干预3 d、7 d后3组小鼠植入体附近组织匀浆中MRSA数量,并通过酶联免疫吸附实验(ELISA)测定组织匀浆中炎症因子的水平,通过苏木精-伊红染色评估组织炎症感染的程度。结果:在光照条件下,Ti-PGMB植入体能够产生ROS并可消散成熟生物膜。在体内抗感染实验中,干预3d、7d后,Ti-PGMB-L组细菌存活率与Ti组(100%)比较,差异均无统计学意义(P均>0.05);而Ti-PGMB+L组干预3 d、7 d后细菌存活率与Ti组(100%)比较,差异均有统计学意义(P均<0.05)。干预7 d后,Ti-PGMB+L组组织匀浆中的白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)炎症因子水平低于Ti-PGMB-L组和Ti组,差异均有统计学意义(P均<0.05)。结论:Ti-PGMB利用其可调控的光动力效应生成ROS,从而消杀感染性细菌及其形成的生物膜。Ti-PGMB能够抑制以MRSA为代表的植入式医疗器械感染、减少炎症反应,具有重要的临床价值。
Objective:To evaluate the anti-infection performance of aminomethyl blue modified poly glycidyl methacrylate modified titanium implant(Ti-PGMB).Methods:A fluorescent probe was used to confirm the production of reactive oxygen species(ROS)by Ti-PGMB implant under light irradiation.To further evaluate the antibacterial effectiveness of Ti-PGMB,MRSA biofilm models and animal models with implant-related infections were established.Mice were randomly divided into three groups:Ti implant control(Ti),Ti-PGMB without light irradiation(Ti-PGMB-L),and Ti-PGMB with light irradiation(Ti-PGMB+L),with 10 mice in each group.In the Ti group,titanium tablets were implanted,while the Ti-PGMB was implanted in the Ti-PGMB+L and Ti-PGMB-L groups.MRSA bacterial suspensions(5×10^(7) CFU/mL)were inoculated at the implantation sites.After 24 hours,the Ti-PGMB+L group received continuous light intervention for 7 days,while no additional interventions were performed in the Ti and Ti-PGMB-L groups.The recovery of the incisions at the implant site and the number of MRSA in the tissue homogenates near the implant were assessed on days 3 and 7 after intervention.The levels of inflammatory factors in tissue homogenates were measured by enzyme linked immunosorbent assay,and tissue inflammation was assessed by hematoxylin-eosin staining.Results:Under light irradiation,Ti-PGMB implants can generate ROS and dissipate mature biofilms.In vivo anti-infection experiments revealed the survival rates of bacteria in the Ti-PGMB-L group were 97.7%and 98.9%at days 3 and 7 after intervention,respectively,which was comparable to the Ti group(100%)(all P>0.05).In contrast,the survival rates of bacteria were 47.1%and 7.0%in the Ti-PGMB+L group at days 3 and 7 after intervention,respectively,which were statistically significantly lower compared to the Ti group(100%)(all P<0.05).At day 7 after intervention,the levels of the inflammatory factors interleukin-1β,interleukin-6,and tumor necrosis factor-αin tissue homogenates of the Ti-PGMB+L group were significant
作者
聂井君
高敏政
朱艺文
俞丙然
陈大福
NIE Jingjun;GAO Minzheng;ZHU Yiwen;YU Bingran;CHEN Dafu(National Center for Orthopedics,Beijing Research Institute of Traumatology and Orthopedics,Beijing Jishuitan Hospital,Capital Medical University,Beijing 100035,China;Beijing Laboratory of Biomedical Materials,College of Materials Science&Engineering,Beijing University of Chemical Technology,Beijing 100029,China)
出处
《中华骨与关节外科杂志》
CSCD
北大核心
2024年第7期641-647,共7页
Chinese Journal of Bone and Joint Surgery
基金
国家自然科学基金(52293382,52303175)
博士后创新人才支持计划(BX20230035)
中国博士后科学基金面上项目(2022M720377)。
关键词
光动力抗菌
表面功能化
骨科植入体
Photodynamic Antibacterial
Surface Modification
Orthopedic Implants
