摘要
CytoQuan^(®)便携式流式细胞仪是一种新的活菌快速计数设备。本研究以GB 4789.2—2022《食品安全国家标准食品微生物学检验菌落总数测定》为参照方法,依据ISO 16140-2:2016《食物链的微生物学方法验证第2部分:参考法替代(专有)方法验证协议》和GB 4789.45—2023《食品安全国家标准微生物检验方法验证通则》,对CytoQuan^(®)便携式流式细胞仪的活菌计数性能进行验证,包括仪器的线性和检出限、精密度、包容性,并通过检测水、乳酸菌、环境涂抹、食品等样品验证其快速检测效果。结果显示,CytoQuan^(®)便携式流式细胞仪的活菌计数结果与国标法计数结果无显著性差异,定量范围为10~4~10~7 CFU/mL。结果表明,CytoQuan^(®)便携式流式细胞仪可有效快速地计数微生物数量,且与国标法微生物检测相比,流程简单,耗时短,可提高检测效率,满足样品快速检测的需要。
The CytoQuan^(®)mobile flow cytometer is a novel device for rapid counting of viable bacteria.This study aimed to validate the performance of the CytoQuant mobile flow cytometer in viable cell counting,using National Food Safety Standard-Food Microbiological Examination:Aerobic Plant Count(GB 4789.2-2022)as the reference method.The validation was conducted in accordance with Microbiology of the Food Chain-Method Validation-Part 2:Protocol for the Validation of Alternative(Proprietary)Methods against a Reference Method(ISO 16140-2:2016)and National Food Safety Standard-General Rules for Validation of Microbiological Test Methods(GB 4789.45-2023).The validation included assessment of the instrument’s linearity and detection limit,precision,inclusivity,and its rapid detection effectiveness was verified through the examination of samples such as water,lactic acid bacteria,environmental swabs,and food.The results showed that there was no significant difference between the cell counts obtained by the CytoQuant mobile flow cytometer and those by the national standard method,with a quantitative range of 104 to 107 CFU/mL.The findings indicate that the CytoQuant mobile flow cytometer can effectively and rapidly count microbial numbers,and compared with the national standard method for microbial detection,it has a simpler procedure,shorter time consumption,and can improve detection efficiency,meeting the needs for rapid sample detection.
作者
吴丽娜
殷培军
武茂苍
王小叶
段效辉
杨爽
唐静
贾俊涛
WU Li-Na;YIN Pei-Jun;WU Mao-Cang;WANG Xiao-Ye;DUAN Xiao-Hui;YANG Shuang;TANG Jing;JIA Jun-Tao(Technology Center of Qingdao Customs District,Qingdao 266109;Qingdao Dagang Customs,Qingdao 266000;Yantai Customs,Yantai 264000;Tianjin Customs Animal,Plant and Food Testing Center,Tianjin 300000)
出处
《中国口岸科学技术》
2024年第8期91-96,共6页
China Port Science and Technology
基金
海关总署科研项目(2024HK004)。
关键词
流式细胞仪
验证
快速检测
flow cytometry
validation
rapid detection
