摘要
目的:探讨黄芪多糖(APS)调节PI3K/AKT/mTOR信号通路对良性前列腺增生(BPH)大鼠细胞凋亡的影响。方法:通过摘除双侧睾丸联合皮下注射丙酸睾酮建立BPH大鼠模型,并将建模成功的大鼠随机分为BPH组、APS低剂量(APS-低)组(100 mg/kg APS)、APS中剂量(APS-中)组(200 mg/kg APS)、APS高剂量(APS-高)组(400 mg/kg APS)、APS-高+PI3K激活剂-胰岛素生长因子1(IGF-1)组(400 mg/kg APS+5 mg/mL IGF-1),干预结束后,电子天平称量前列腺湿质量,计算前列腺指数;TUNEL染色检测前列腺组织细胞凋亡变化;免疫组化检测凋亡基因Caspase-3表达;Western blotting检测通路相关蛋白及凋亡基因Bcl-2、Bax表达。结果:与假手术组比较,BPH组前列腺组织病理损伤严重,前列腺湿质量及指数、p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR、Bcl-2蛋白表达显著增加,凋亡指数、Caspase-3阳性表达、Bax表达显著降低(P<0.05);与BPH组比较,APS-低组、APS-中组、APS-高组病理损伤改善,前列腺湿质量及指数、p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR、Bcl-2蛋白表达显著降低,凋亡指数、Caspase-3阳性表达、Bax表达显著增加,不同剂量APS比较差异有统计学意义(P<0.05);与APS-高组比较,APS-高+IGF-1组病理损伤稍加重,前列腺湿质量及指数、p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR、Bcl-2蛋白表达显著增加,凋亡指数、Caspase-3阳性表达、Bax表达显著降低(P<0.05)。结论:APS可通过抑制PI3K/AKT/mTOR信号通路促进BPH大鼠细胞凋亡,有效抑制BPH进展。
Objective:To investigate the effect of Astragalus polysaccharides(APS)on cell apoptosis in rats with benign prostatic hyperplasia(BPH)by regulating the PI3K/AKT/mTOR signaling pathway.Methods:A BPH rat model was established by bilateral testicular removal combined with subcutaneous injection of testosterone propionate.The successfully modeled rats were randomly separated into BPH group,APS low dose(APS low)group(100 mg/kg APS),APS medium dose(APS medium)group(200 mg/kg APS),APS high dose(APS high)group(400 mg/kg APS),and APS high dose+PI3K activator insulin growth factor 1(IGF-1)group(400 mg/kg APS+5 mg/mL IGF-1).After the intervention,an electronic balance was used to weigh the wet weight of the prostate,and the prostate index was calculated.TUNEL staining was applied to detect changes in apoptosis of prostate tissue cells.Immunohistochemistry was applied to detect the expression of the apoptotic gene Caspase-3.Western blotting was applied to detect the expression of pathway related proteins and apoptotic genes Bcl-2 and Bax.Results:Compared with the sham surgery group,the BPH group showed severe pathological damage to the prostate tissue.The prostate wet weight and index,p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR,and Bcl-2 protein expression greatly increased in BPH group,while the apoptosis index,Caspase-3 positive expression,and Bax expression greatly reduced in BPH group(P<0.05).Compared with the BPH group,the pathological damage was improved in the APS low group,APS medium group,and APS high group.The prostate wet weight and index,p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR,and Bcl-2 protein expression reduced in APS low group,APS medium group,and APS high group,while the apoptosis index,Cacpase-3 positive expression,and Bax expression increased greatly in APS low group,APS medium group,and APS high group.There was statistical difference among different doses of APS(P<0.05).Compared with the APS high group,the pathological damage was slightly aggravated in APS high+IGF-1 group.The prostate wet weight and index,p-PI3K/PI3K,p-A
作者
赵猛
孙一诺
齐爽
郑雪
ZHAO Meng;SUN Yinuo;QI Shuang;ZHENG Xue(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150040,China;Heilongjiang Provincial Traditional Chinese Medicine Hospital,Harbin Heilongjiang 150000,China;Harbin Jingen Kidney Disease Specialized Hospital,Harbin Heilongjiang 150036,China)
出处
《中医药导报》
2024年第8期22-26,33,共6页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
黑龙江省卫生健康委科研课题(20220404051077)。
