摘要
【目的】研究新疆野生巴尔喀什蘑菇子实体粗多糖提取及多糖脱色工艺,为新疆野生巴尔喀什蘑菇多糖的纯化及深度开发提供理论依据和技术支持。【方法】采用热水提醇沉法提取巴尔喀什蘑菇子实体粗多糖,研究提取温度、时间、次数对巴尔喀什蘑菇多糖得率的影响;选择脱色时间、脱色温度、活性炭和H_(2)O_(2)含量为影响因子,以多糖脱色率为研究指标,采用正交试验优化巴尔喀什蘑菇多糖脱色工艺,确定最佳脱色工艺条件。【结果】热水提醇沉法提取巴尔喀什菇子实体粗多糖的最佳工艺条件:提取时间6 h、提取温度60℃、提取次数2次,在此条件下巴尔喀什菇子实体粗多糖得率为15.6%。活性炭最佳脱色工艺为活性炭用量25%、脱色温度60℃、脱色时间45 min、pH值3,在此条件下脱色率为80.4%;双氧水最佳脱色工艺为H_(2)O_(2)含量10%、脱色温度60℃、脱色时间3 h、pH值9,在此条件下脱色率为86.23%。【结论】利用热水提醇沉法提取巴尔喀什蘑菇粗多糖工艺具有实际应用价值。H_(2)O_(2)脱色法和活性炭脱色法对巴尔喀什蘑菇多糖的脱色效果受不同工艺条件的影响差异较大,H_(2)O_(2)脱色率比活性炭脱色率高,是巴尔喀什蘑菇多糖脱色的可行方法。
【Objective】To study the extraction and decolorization process of crude polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang,and provide theoretical basis and technical support for its in-depth development.【Methods】This study adopted ethanol subsiding to extract the fruit body polysaccharides of wild A.balchaschensisin,and explored the influence of extraction temperature,time and frequency on the yield of polysaccharides from A.balchaschensisin.With the decolorization time,decolorization temperature,content of activated carbon and H_(2)O_(2)as influencing factors and the decolorization rate of polysaccharides as research index,the study optimized the decolorization process of polysaccharides from A.balchaschensisin through orthogonal test,so as to determine the optimal decolorization conditions.【Results】According to the results,the optimal conditions for extracting fruit body polysaccharides of wild A.balchaschensis by ethanol subsiding were 6 h extraction time,60℃extraction temperature,and 2 times extraction,under which the yield of fruit body polysaccharides of wild A.balchaschensisin was 15.6%.The optimal decolorization process for activated carbon was 25%activated carbon,60℃decolorization temperature,and 45 min decolorization time at pH3,under which the decolorization rate was 80.4%.The optimal decolorization process for hydrogen peroxide was 10%H_(2)O_(2)content,60℃decolorization temperature,and 3 h decolorization time at pH 9,under which the decolorization rate was 86.23%.【Conclusion】This technology has verified the feasibility and practical value of applying ethanol subsiding to extract fruit body polysaccharides of wild A.balchaschensis in and the effects of H_(2)O_(2)and activated carbon decolorization methods on the decolorization of polysaccharides from A.balchaschensis varied greatly under different process conditions.The former has a higher decolorization rate than the latter and is feasible for the decolorization of polysaccharides from A.balchaschensisin.
作者
努尔孜亚·亚力买买提
罗影
贾培松
郝敬喆
贾文捷
朱琦
梁志豪
蔺思茹
赵振豪
Nuerziya Yalimaimaiti;LUO Ying;JIA Peisong;HAO Jingzhe;JIA Wenjie;ZHU Qi;LIANG Zhihao;LIN Siru;ZHAO Zhenhao(Key Laboratory of Integrated Crop Pest Management on Crop in Northwestern Oasis,Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;College of Agricalture Xinjiang Agriculture University,Urumqi 830052,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2024年第6期1527-1534,共8页
Xinjiang Agricultural Sciences
基金
国家自然科学基金地区基金项目(32060709,31560577)
现代农业产业技术体系建设专项资金(CARS-20)。
