摘要
目的 探讨传统南药巴戟天抗疲劳组分与药效关系的相关性,为其物质基础研究及质量标准完善提供研究基础。方法 采用HPLC法建立巴戟天抗疲劳相关组分指纹图谱;采用小鼠负重游泳实验评价受试物抗疲劳药效;采用灰色关联度分析法(GRA法)及偏最小二乘回归分析法(PLSR法)分析不同批次巴戟天抗疲劳相关组分与其抗疲劳药效相关性。结果 不同批次巴戟天测出的组分有水晶兰苷、去乙酰基车叶草苷酸、2-甲基蒽醌、甲基异茜草素、去乙酰车叶草苷酸甲酯、车叶草苷及甘草素,其中水晶兰苷含量明显高于其余组分;GRA法分析显示以上组分与抗疲劳药效对应的关联度分别为0.883 5、0.839 3、0.835 9、0.807 4、0.791 6、0.735 1及0.704 9;PLSR法分析显示与抗疲劳药效正相关的组分按变量重要性投影值(VIP)大小排序为去乙酰车叶草苷酸甲酯、去乙酰基车叶草苷酸、水晶兰苷、2-甲基蒽醌,前三组分VIP值远大于2-甲基蒽醌且基本接近。结论 巴戟天抗疲劳药效为多种成分共同作用的结果,综合GRA法及PLSR法结果,对抗疲劳作用相关性较大的为水晶兰苷、去乙酰基车叶草苷酸及去乙酰车叶草苷酸甲酯,其中水晶兰苷相关性较大且含量最高,可考虑作为抗疲劳活性特征目标组分,深入研究其作为含量测定指标成分的可行性。
OBJECTIVE To explore the correlation between anti-fatigue components of traditional southern medicine Morinda oficinalis and drug efficacy,and to provide research basis for its active ingredients study and quality standard improvement.METHODS The fingerprint of anti-fatigue components of Morinda officinalis was established by HPLC.The anti-fatigue effect of the tested substance was evaluated by the mouse weight-bearing swimming experiment.The correlation between anti-fatigue components and anti-fatigue effects of Morinda officinalis from different batches was analyzed by grey correlation degree Analysis(GRA)and partial least squares regression analysis(PLSR).RESULTS The components detected from different batches of Morinda officinalis were monotropein,Deacetylasperulosidic acid,2-methylanthraquinone,methyl isoalizarin,Deacetylasperulosidic acid methyl ester,aglycoside and liquiritin.The content of monotropein was significantly higher than that of other components.GRA analysis showed that the correlations degreesof the above components and anti fatigue efficacy were 0.8835,0.8393,0.8359,0.8074,0.7916,0.7351 and 0.7049,respectively.PLSR analysis showed that the components positively correlated with the anti-fatigue efficacy were Deacetylasperulosidic acid methyl ester,Deacetylasperulosidic acid,monotropein and 2-methyl-anthraquinone according to the magnitude of variable importance projection(VIP).The VIP values of the first three components were much higher than that of 2-methyl-anthraquinone and almost similar.CONCLUSION The anti-fatigue efficacy of Morinda oficinarum is the result of theco-action of several components.Based on the results of grey correlation analysis and partial least squares regression analysis,the most correlated anti-fatigue effects were monotropein,Deacetylasperulosidic acid,and Deacetylasperulosidic acid methyl ester,among which monotropein was correlated and had the highest content.Therefore,monotropein can be considered as the characteristic target component of anti-fatigue activity,and t
作者
何华红
伍良涌
许俊豪
莫韵斯
李玮玲
庞智慧
谢美贤
李薇
HE Huahong;WU Liangyong;XU Junhao;MO Yunsi;LI Weiling;PANG Zhihui;XIE Meixian;LI Wei(Guangzhou Institute for Drug Control,Guangzhou,Guangdong 510160,China)
出处
《今日药学》
CAS
2024年第8期572-577,共6页
Pharmacy Today
基金
广州市科技计划资助项目(202201010843)。
关键词
巴戟天
谱效研究
抗疲劳
灰色关联度
偏最小二乘回归分析法
Morinda oficinalis
spectral efficiency research
anti fatigue
grey correlation degree
partial least squares regression analysis
