摘要
建立液相色谱法检测特殊医学用途婴幼儿配方食品中VC含量的分析方法。样品还原处理后,经HLB固相萃取柱净化、微孔滤膜过滤后进行亲水作用液相色谱法测定,外标法定量,对检测方法进行验证。结果表明:在质量浓度为0~50μg/mL范围内其线性相关系数≥0.999,定量限为3.0 mg/100 g,加标回收率为95.2%~102.8%,相对标准偏差为1.22%~3.62%;该方法具有操作简单、结果准确和重复性好等特点,适用于特殊医学用途婴幼儿配方食品中VC含量的测定。
A liquid chromatography(LC)method for the determination of vitamin C in formulas for special medical purpose intended for infants was established and validated.The sample was reduced,purified using an HLB solid phase extraction(SPE)cartridge,filtered through a 0.45μm filter,and analyzed by hydrophilic interaction liquid chromatography(HILIC).The calibration curve was found to be linear in the concentration range of 0–50μg/mL with correlation coefficient over 0.999.The limit of quantitation(LOQ)was 3.0 mg/100 g.Recoveries of spiked samples ranged from 95.2%to 102.8%with relative standard deviation(RSD)between 1.22%and 3.62%.The method is characterized by simple operation,accurate results and good reproducibility and suitable to determine vitamin C in formulas for special medical purpose intended for infants.
作者
吴秀萍
程白羽
许小茜
姜睿彧
WU Xiuping;CHENG Baiyu;XU Xiaoqian;JIANG Ruiyu(Shanghai Institute of Quality Inspection and Technical Research,Shanghai 200233,China)
出处
《乳业科学与技术》
2024年第3期35-40,共6页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
关键词
特殊医学用途婴幼儿配方食品
VC
液相色谱法
亲水作用液相色谱
formulas for special medical purpose intended for infants
vitamin C
liquid chromatography
hydrophilic interaction liquid chromatography
