摘要
目的:线粒体翻译延长因子Tu(mitochondrial Tu translation elongation factor,TUFM)已被证明参与秦川牛宰后成熟过程中的细胞自噬活动,实验探究该过程中TUFM表达与能量代谢变化的关系。方法:以秦川牛背最长肌为研究对象,测定4℃不同成熟时间(0、2、12、24、48、96、144、192 h)TUFM表达量及含量、葡萄糖(glucose,GLU)、乳酸(lactic acid,LA)、腺苷三磷酸(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、单磷酸腺苷(adenosine monophosphate,AMP)6种物质含量以及乳酸脱氢酶(lactate dehydrogenase,LDH)、琥珀酸脱氢酶(succinate dehydrogenase,SDH)、磷酸丙糖异构酶(triose phosphate isomerase,TPI)、苹果酸脱氢酶(malate dehydrogenase,MDH)、细胞色素c氧化酶(cytochrome c oxidase,COX)5种酶活性的变化情况。结果:在秦川牛宰后成熟期间,GLU、ATP、ADP、AMP含量和LDH、SDH、TPI活性均呈下降趋势,TUFM表达量、MDH活性及LA和TUFM含量均呈先上升后下降趋势,COX活性呈先下降后上升再下降趋势。能量代谢各指标和TUFM蛋白主要在宰后初期发挥作用,对宰后初期及宰后中后期分别进行Pearson相关性分析,结果表明,在宰后初期牛背最长肌中TUFM表达量与MDH、LA呈极显著正相关(P<0.01),与ATP、ADP、AMP、LDH、SDH、TPI、COX、GLU呈极显著负相关(P<0.01)。在宰后中后期,TUFM表达与所有指标(GLU、LA、ATP、ADP、AMP、LDH、MDH、SDH、TPI、COX)均呈极显著正相关(P<0.01)。结论:在宰后初期TUFM表达量逐渐增加,可为肌肉细胞提供能量从而用于能量代谢途径,该过程可能是TUFM正向参与细胞自噬所致。在宰后中后期能量短缺时,TUFM可能抑制细胞自噬,优先将能量用于除细胞自噬外的其他重要途径(如能量代谢途径)以维持细胞稳态。综上,在宰后成熟过程中TUFM具有双重作用,可调节细胞自噬为能量代途径提供能量,有助于维持宰后能量代谢持续的时间。
Mitochondrial Tu translation elongation factor(TUFM)has been demonstrated to be involved in cellular autophagy during the postmortem aging of Qinchuan beef.This study aimed to explore the relationship between TUFM expression and changes in energy metabolism during this process.Changes in the expression and content of TUFM,the contents of glucose(GLU),lactic acid(LA),adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),and the activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),triose phosphate isomerase(TPI),malate dehydrogenase(MDH),and cytochrome c oxidase(COX)in the Longissimus dorsi muscle of Qinchuan cattle were determined at different periods(0,2,12,24,48,96,144 and 192 h)of aging at 4℃.The results showed that during postmortem aging,the contents of GLU,ATP,ADP and AMP,and the activities of LDH,SDH and TPI exhibited a decreasing trend.Meanwhile,the expression level of TUFM,MDH activity,and the contents of LA and TUFM showed an initial increase followed by a decrease.The activity of COX exhibited an initial decrease followed by a temporary increase and then a decrease.The various indicators of energy metabolism and TUFM exerted their effects mainly at the early stage of postmortem aging.Pearson’s correlation analysis indicated that the expression level of TUFM was significantly positively correlated with MDH and LA but significantly negatively correlated with ATP,ADP,AMP,LDH,SDH,TPI,COX,and GLU at the early stage(P<0.01).At the middle and late stages,TUFM expression was significantly positively correlated with all these indicators(GLU,LA,ATP,ADP,AMP,LDH,MDH,SDH,TPI and COX)(P<0.01).In conclusion,during the early period of postmortem aging,the gradual increase of TUFM expression can provide energy to muscle cells for energy metabolism pathways,which may be possibly due to the positive involvement of TUFM in cellular autophagy.When energy is deficient during the middle and late periods,TUFM may inhibit cellular autophagy to prioritize energy use for importan
作者
司健芳
高爽
张静
李亚蕾
罗瑞明
SI Jianfang;GAO Shuang;ZHANG Jing;LI Yaei;LUO Ruiming(School of Food Science and Engineering,Ningxia University,Yinchuan 750021,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第17期1-8,共8页
Food Science
基金
国家自然科学基金地区科学基金项目(32160535)。
关键词
线粒体翻译延长因子Tu
能量代谢
秦川牛
细胞自噬
mitochondrial Tu translation elongation factor
energy metabolism
Qinchuan cattle
cellular autophagy
