摘要
目的了解广州地区副溶血性弧菌的血清型、毒力基因和分子特征,为副溶血性弧菌疾病的防控提供科学依据。方法对2020-2022年收集到的副溶血性弧菌菌株进行血清分型、毒力基因检测和脉冲场凝胶电泳(PFGE),并使用国家致病菌识别网的比对功能进行同源性分析。结果收集到132株副溶血性弧菌,其中50.8%(67/132)的菌株血清型为O10:K4,其次为O3:K6(34.8%,46/132);毒力基因结果显示所有的菌株tlh基因阳性(100.0%,132/132),有130株tdh基因阳性(98.5%,130/132),2株trh基因阳性(1.5%,2/132);PFGE聚类分析发现菌株可分为31个PFGE型别,菌株间相似值为23.54%~100.00%,有6个集中聚类。结论广州地区副溶血性弧菌引起的食源性疾病以O10:K4和O3:K6血清型为主,大多数菌株携带tdh基因,PFGE图谱提示基因多样性。
Objective To analyze the serotype,virulence genes and gene characteristics of Vibrio parahaemolyticus isolates from Guangzhou,and provide a scientific basis for prevention and control of Vibrio parahaemolyticus.Methods The serotypes,virulence genes and pulse⁃field gel electrophoresis(PFGE)type of all the isolated stains of Vibrio parahaemolyticus were detected during 2020-2022.Homology analysis was performed using the comparison function of the Chinese Pathogen Identification Net.Results O10:K4(50.8%,67/132)and O3:K6(34.8%,46/132)were the predominant serotypes of the 132 Vibrio parahaemolyticus strains isolated.Virulence gene detection indicated all strains were detected positive for tlh genes(100.0%,132/132),130 isolates expressed tdh genes(98.5%,130/132),only 2 isolates expressed trh genes(1.5%,2/132).These strains could be divided into 31 PFGE patterns,and the similarity coefficients were between 23.54%-100.00%.It belonged to 6 clone clusters.Conclusions O10:K4 and O3:K6 were the most dominant serotypes in Vibrio parahaemolyticus strains from foodborne disease in Guangzhou,and tdh was detected in most of the strains.PFGE showed there was great diversity in this gene.
作者
陈佳璇
刘巧谊
张晶
陶霞
陈惠玲
张欣强
CHEN Jiaxuan;LIU Qiaoyi;ZHANG Jing;TAO Xia;CHEN Huiling;ZHANG Xinqiang(Guangzhou Center for Disease Control and Prevention,Guangzhou,Guangdong 510440,China)
出处
《热带医学杂志》
CAS
2024年第7期1036-1039,共4页
Journal of Tropical Medicine
基金
广东省医学科学技术研究基金(A2021040)
广州市医学重点学科
广州市科技计划项目(2024A03J0420)。
关键词
副溶血性弧菌
血清型
毒力基因
脉冲场凝胶电泳
Vibrio parahaemolyticus
Serotypes
Virulence gene
Pulse⁃field gel electrophoresis
